CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002124
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Glutathione synthetase 
Protein Synonyms/Alias
 GSH synthetase; GSH-S; GSHase; Glutathione synthase 
Gene Name
 gshB 
Gene Synonyms/Alias
 gsh-II; b2947; JW2914 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
17PIANINIKKDSSFAMacetylation[1]
108ILERAEEKGTLIVNKacetylation[1]
115KGTLIVNKPQSLRDCacetylation[1]
144ETLVTRNKAQLKAFWacetylation[1]
148TRNKAQLKAFWEKHSacetylation[1]
153QLKAFWEKHSDIILKacetylation[1]
160KHSDIILKPLDGMGGacetylation[1]
174GASIFRVKEGDPNLGacetylation[1]
205QNYLPAIKDGDKRVLacetylation[1]
253PLTESDWKIARQIGPacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
  
Sequence Annotation
 DOMAIN 125 310 ATP-grasp.
 NP_BIND 151 207 ATP (By similarity).
 METAL 281 281 Magnesium or manganese (By similarity).
 METAL 283 283 Magnesium or manganese (By similarity).  
Keyword
 3D-structure; ATP-binding; Complete proteome; Direct protein sequencing; Glutathione biosynthesis; Ligase; Magnesium; Manganese; Metal-binding; Nucleotide-binding; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 316 AA 
Protein Sequence
MIKLGIVMDP IANINIKKDS SFAMLLEAQR RGYELHYMEM GDLYLINGEA RAHTRTLNVK 60
QNYEEWFSFV GEQDLPLADL DVILMRKDPP FDTEFIYATY ILERAEEKGT LIVNKPQSLR 120
DCNEKLFTAW FSDLTPETLV TRNKAQLKAF WEKHSDIILK PLDGMGGASI FRVKEGDPNL 180
GVIAETLTEH GTRYCMAQNY LPAIKDGDKR VLVVDGEPVP YCLARIPQGG ETRGNLAAGG 240
RGEPRPLTES DWKIARQIGP TLKEKGLIFV GLDIIGDRLT EINVTSPTCI REIEAEFPVS 300
ITGMLMDAIE ARLQQQ 316 
Gene Ontology
 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IEA:HAMAP.
 GO:0004363; F:glutathione synthase activity; IDA:EcoCyc.
 GO:0000287; F:magnesium ion binding; IDA:EcoCyc.
 GO:0030145; F:manganese ion binding; IEA:HAMAP. 
Interpro
 IPR011761; ATP-grasp.
 IPR013815; ATP_grasp_subdomain_1.
 IPR013816; ATP_grasp_subdomain_2.
 IPR006284; Glut_synth_pro.
 IPR004218; GSHS_ATP-bd.
 IPR004215; GSHS_N.
 IPR016185; PreATP-grasp_dom. 
Pfam
 PF02955; GSH-S_ATP
 PF02951; GSH-S_N 
SMART
  
PROSITE
 PS50975; ATP_GRASP 
PRINTS