CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004197
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 60S ribosomal protein L3 
Protein Synonyms/Alias
 Maintenance of killer protein 8; RP1; Trichodermin resistance protein; YL1 
Gene Name
 RPL3 
Gene Synonyms/Alias
 MAK8; TCM1; YOR063W; YOR29-14 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
34ARVKAFPKDDRSKPVubiquitination[1]
39FPKDDRSKPVALTSFacetylation[2]
39FPKDDRSKPVALTSFubiquitination[1, 3]
50LTSFLGYKAGMTTIVubiquitination[1, 3]
66DLDRPGSKFHKREVVacetylation[2]
120EVKRRFYKNWYKSKKubiquitination[1]
132SKKKAFTKYSAKYAQacetylation[2]
136AFTKYSAKYAQDGAGacetylation[2]
136AFTKYSAKYAQDGAGubiquitination[1, 3]
153RELARIKKYASVVRVubiquitination[1]
168LVHTQIRKTPLAQKKubiquitination[1]
191NGGSISEKVDWAREHubiquitination[1]
201WAREHFEKTVAVDSVubiquitination[1]
222IDAIAVTKGHGFEGVubiquitination[1]
248KTHRGLRKVACIGAWubiquitination[1]
281SRTSINHKIYRVGKGacetylation[2]
281SRTSINHKIYRVGKGubiquitination[1]
287HKIYRVGKGDDEANGubiquitination[1]
303TSFDRTKKTITPMGGubiquitination[1]
318FVHYGEIKNDFIMVKubiquitination[1]
340KRIVTLRKSLYTNTSubiquitination[1]
349LYTNTSRKALEEVSLubiquitination[1]
357ALEEVSLKWIDTASKacetylation[2]
357ALEEVSLKWIDTASKubiquitination[1]
364KWIDTASKFGKGRFQacetylation[2]
364KWIDTASKFGKGRFQubiquitination[1]
376RFQTPAEKHAFMGTLacetylation[2]
376RFQTPAEKHAFMGTLubiquitination[1]
384HAFMGTLKKDL****acetylation[2]
384HAFMGTLKKDL****methylation[4]
384HAFMGTLKKDL****ubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [3] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [4] Identification of arginine- and lysine-methylation in the proteome of Saccharomyces cerevisiae and its functional implications.
 Pang CN, Gasteiger E, Wilkins MR.
 BMC Genomics. 2010 Feb 5;11:92. [PMID: 20137074] 
Functional Description
  
Sequence Annotation
 MOD_RES 24 24 Phosphoserine.
 MOD_RES 103 103 Phosphothreonine.
 MOD_RES 243 243 Pros-methylhistidine.
 MOD_RES 297 297 Phosphoserine.  
Keyword
 3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; Methylation; Phosphoprotein; Reference proteome; Ribonucleoprotein; Ribosomal protein. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 387 AA 
Protein Sequence
MSHRKYEAPR HGHLGFLPRK RAASIRARVK AFPKDDRSKP VALTSFLGYK AGMTTIVRDL 60
DRPGSKFHKR EVVEAVTVVD TPPVVVVGVV GYVETPRGLR SLTTVWAEHL SDEVKRRFYK 120
NWYKSKKKAF TKYSAKYAQD GAGIERELAR IKKYASVVRV LVHTQIRKTP LAQKKAHLAE 180
IQLNGGSISE KVDWAREHFE KTVAVDSVFE QNEMIDAIAV TKGHGFEGVT HRWGTKKLPR 240
KTHRGLRKVA CIGAWHPAHV MWSVARAGQR GYHSRTSINH KIYRVGKGDD EANGATSFDR 300
TKKTITPMGG FVHYGEIKND FIMVKGCIPG NRKRIVTLRK SLYTNTSRKA LEEVSLKWID 360
TASKFGKGRF QTPAEKHAFM GTLKKDL 387 
Gene Ontology
 GO:0022625; C:cytosolic large ribosomal subunit; TAS:SGD.
 GO:0003735; F:structural constituent of ribosome; TAS:SGD.
 GO:0002181; P:cytoplasmic translation; TAS:SGD.
 GO:0000027; P:ribosomal large subunit assembly; IMP:SGD. 
Interpro
 IPR000597; Ribosomal_L3.
 IPR019926; Ribosomal_L3_CS.
 IPR009000; Transl_elong_init/rib_B-barrel. 
Pfam
 PF00297; Ribosomal_L3 
SMART
  
PROSITE
 PS00474; RIBOSOMAL_L3 
PRINTS