CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-037231
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Serine/threonine-protein kinase Nek4 
Protein Synonyms/Alias
  
Gene Name
 NEK4 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
14CYLRVVGKGSYGEVTubiquitination[1]
44HILHRDLKTQNVFLTubiquitination[1]
57LTRTNIIKVGDLGIAubiquitination[1]
120LKHAFNAKDMNSLVYubiquitination[1]
133VYRIIEGKLPPMPRDubiquitination[1]
184SFFLEATKIKTSKNNubiquitination[1]
200KNGDSQSKPFATVVSubiquitination[1]
250PRASGLLKSPASLKAubiquitination[1]
520SSEMSSSKDRPLSARubiquitination[1]
632QTLKLDSKESCEDVPubiquitination[1]
673EAEEIHFKELPSAIMubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 722 AA 
Protein Sequence
MPLAAYCYLR VVGKGSYGEV TLVKHRRDGK QYLHEKHILH RDLKTQNVFL TRTNIIKVGD 60
LGIARVLENH CDMASTLIGT PYYMSPELFS NKPYNYKSDV WALGCCVYEM ATLKHAFNAK 120
DMNSLVYRII EGKLPPMPRD YSPELAELIR TMLSKRPEER PSVRSILRQP YIKRQISFFL 180
EATKIKTSKN NIKNGDSQSK PFATVVSGEA ESNHEVIHPQ PLSSEGSQTY IMGEGKCLSQ 240
EKPRASGLLK SPASLKAHTC KQDLSNTTEL ATISSVNIDI LPAKGRDSVS DGFVQENQPR 300
YLDASNELGG ICSISQVEEE MLQDNTKSSA QPENLIPMWS SDIVTGEKNE PVKPLQPLIK 360
EQKPKDQSLA LSPKLECSGT ILAHSNLRLL GSSDSPASAS RVAGITGVCH HAQDQVAGEC 420
IIEKQGRIHP DLQPHNSGSE PSLSRQRRQK RREQTEHRGE KRQVRRDLFA FQESPPRFLP 480
SHPIVGKVDV TSTQKEAENQ RRVVTGSVSS SRSSEMSSSK DRPLSARERR RLKQSQEEMS 540
SSGPSVRKAS LSVAGPGKPQ EEDQPLPARR LSSDCSVTQE RKQIHCLSED ELSSSTSSTD 600
KSDGDYGEGK GQTNEINALV QLMTQTLKLD SKESCEDVPV ANPVSEFKLH RKYRDTLILH 660
GKVAEEAEEI HFKELPSAIM PGSEKIRRLV EVLRTDVIRG LGVQLLEQVY DLLEEEDEFD 720
RE 722 
Gene Ontology
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0005524; F:ATP binding; IEA:InterPro.
 GO:0004674; F:protein serine/threonine kinase activity; IEA:InterPro. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00069; Pkinase 
SMART
  
PROSITE
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS