CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004262
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Carbamoyl-phosphate synthase large chain 
Protein Synonyms/Alias
 Carbamoyl-phosphate synthetase ammonia chain 
Gene Name
 carB 
Gene Synonyms/Alias
 STM0067 
Created Date
 July 27, 2013 
Organism
 Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) 
NCBI Taxa ID
 99287 
Lysine Modification
Position
Peptide
Type
References
504FADARLAKLAGVREAacetylation[1]
Reference
 [1] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786
Functional Description
  
Sequence Annotation
 DOMAIN 133 328 ATP-grasp 1.
 DOMAIN 679 870 ATP-grasp 2.
 NP_BIND 159 216 ATP (By similarity).
 NP_BIND 705 762 ATP (By similarity).
 REGION 2 403 Carboxyphosphate synthetic domain.
 REGION 404 553 Oligomerization domain.
 REGION 554 936 Carbamoyl phosphate synthetic domain.
 REGION 937 1075 Allosteric domain.
 METAL 285 285 Magnesium or manganese 1 (By similarity).
 METAL 299 299 Magnesium or manganese 1 (By similarity).
 METAL 299 299 Magnesium or manganese 2 (By similarity).
 METAL 301 301 Magnesium or manganese 2 (By similarity).
 METAL 829 829 Magnesium or manganese 3 (By similarity).
 METAL 841 841 Magnesium or manganese 3 (By similarity).
 METAL 841 841 Magnesium or manganese 4 (By similarity).
 METAL 843 843 Magnesium or manganese 4 (By similarity).  
Keyword
 Amino-acid biosynthesis; Arginine biosynthesis; ATP-binding; Complete proteome; Ligase; Magnesium; Manganese; Metal-binding; Nucleotide-binding; Pyrimidine biosynthesis; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1075 AA 
Protein Sequence
MPKRTDIKSI LILGAGPIVI GQACEFDYSG AQACKALREE GYRVILVNSN PATIMTDPEM 60
ADATYIEPIH WEVVRKIIEK ERPDAVLPTM GGQTALNCAL ELERQGVLEE FGVTMIGATA 120
DAIDKAEDRR RFDIAMKKIG LDTARSGIAH TMEEALAVAA DVGFPCIIRP SFTMGGTGGG 180
IAYNREEFEE ICERGLDLSP TNELLIDESL IGWKEYEMEV VRDKNDNCII VCSIENFDAM 240
GIHTGDSITV APAQTLTDKE YQIMRNASMA VLREIGVETG GSNVQFAVNP KNGRLIVIEM 300
NPRVSRSSAL ASKATGFPIA KVAAKLAVGY TLDELMNDIT GGRTPASFEP SIDYVVTKIP 360
RFNFEKFAGA NDRLTTQMKS VGEVMAIGRT QQESLQKALR GLEVGATGFD PKVSLDDPEA 420
LTKIRRELKD AGADRIWYIA DAFRAGLSVD GVFNLTNIDR WFLVQIEELV RLEEKVAEVG 480
ITGLNADFLR QLKRKGFADA RLAKLAGVRE AEIRKLRDQY DLHPVYKRVD TCAAEFATDT 540
AYMYSTYEDE CEANPSIDRD KIMVLGGGPN RIGQGIEFDY CCVHASLALR EDGYETIMVN 600
CNPETVSTDY DTSDRLYFEP VTLEDVLEIV RIEKPKGVIV QYGGQTPLKL ARALEAAGVP 660
VIGTSPDAID RAEDRERFQH AVDRLKLKQP ANATVTAIEQ AVEKAKEIGY PLVVRPSYVL 720
GGRAMEIVYD EADLRRYFQT AVSVSNDAPV LLDRFLDDAV EVDVDAICDG EMVLIGGIME 780
HIEQAGVHSG DSACSLPAYT LSQEIQDVMR QQVQKLAFEL QVRGLMNVQF AVKDNEVYLI 840
EVNPRAARTV PFVSKATGVP LAKVAARVMA GKSLTEQGVT QEIIPPYYSV KEVVLPFNKF 900
PGVDPLLGPE MRSTGEVMGV GRTFAEAFAK AQLGSNSTMK KQGRALLSVR EGDKERVVDL 960
AAKLLKQGFE LDATHGTAIV LGEAGINPRL VNKVHEGRPH IQDRIKNGEY TYIINTTAGR 1020
RAIEDSRVIR RSALQYKVHY DTTLNGGFAT TMALNADATE KVTSVQEMHA QIKKS 1075 
Gene Ontology
 GO:0005524; F:ATP binding; IEA:HAMAP.
 GO:0004088; F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity; IEA:HAMAP.
 GO:0000287; F:magnesium ion binding; IEA:HAMAP.
 GO:0030145; F:manganese ion binding; IEA:HAMAP.
 GO:0044205; P:'de novo' UMP biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0006526; P:arginine biosynthetic process; IEA:HAMAP. 
Interpro
 IPR011761; ATP-grasp.
 IPR013815; ATP_grasp_subdomain_1.
 IPR013816; ATP_grasp_subdomain_2.
 IPR006275; CarbamoylP_synth_lsu.
 IPR005481; CarbamoylP_synth_lsu_N.
 IPR005480; CarbamoylP_synth_lsu_oligo.
 IPR005479; CbamoylP_synth_lsu-like_ATP-bd.
 IPR005483; CbamoylP_synth_lsu_CPSase_dom.
 IPR011607; MGS-like_dom.
 IPR016185; PreATP-grasp_dom. 
Pfam
 PF00289; CPSase_L_chain
 PF02786; CPSase_L_D2
 PF02787; CPSase_L_D3
 PF02142; MGS 
SMART
 SM01096; CPSase_L_D3
 SM00851; MGS 
PROSITE
 PS50975; ATP_GRASP
 PS00866; CPSASE_1
 PS00867; CPSASE_2 
PRINTS
 PR00098; CPSASE.