UniProt Accession

 RPC4_HUMAN; P05423; Q6FI28; Q9BPV7; Q9BPZ1; Q9BXB3 

Genbank Protein ID

 M17754; AY092086; CR536509; CR541803; BC000516; BC003039; BC002603; BC004484; AF346574; L15301 

Genbank Nucleotide ID

 AAA51838.1; AAM18216.1; CAG38747.1; CAG46602.1; AAH02603.1; AAH04484.1; AAK15371.1; AAA72377.1 

Protein Name

 DNA-directed RNA polymerase III subunit RPC4 

Protein Synonyms/Alias

 RNA polymerase III subunit C4; DNA-directed RNA polymerase III subunit D; Protein BN51; RNA polymerase III 47 kDa subunit; RPC53 homolog 

Gene Name

 POLR3D 

Gene Synonyms/Alias

 BN51; BN51T 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
152	RETDEETKQILRMLE	ubiquitination	[1]
302	DGQVVLIKQEKDREA	ubiquitination	[2]
310	QEKDREAKLAENACT	ubiquitination	[1, 3]
328	LTEGQVGKLLIRKSG	ubiquitination	[1, 3]
382	VLGHVKHKLVCSPDF	ubiquitination	[1]
396	FESLLDHKHR*****	ubiquitination	[1, 3]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

 DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway (By similarity). 

Sequence Annotation

  

Keyword

 Antiviral defense; Complete proteome; DNA-directed RNA polymerase; Immunity; Innate immunity; Nucleus; Reference proteome; Transcription. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 398 AA 

Protein Sequence

Gene Ontology

 GO:0005666; C:DNA-directed RNA polymerase III complex; IEA:InterPro.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0003682; F:chromatin binding; IEA:Compara.
 GO:0003677; F:DNA binding; IEA:InterPro.
 GO:0003899; F:DNA-directed RNA polymerase activity; IEA:UniProtKB-KW.
 GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
 GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
 GO:0045089; P:positive regulation of innate immune response; IDA:UniProtKB.
 GO:0032728; P:positive regulation of interferon-beta production; IDA:UniProtKB.
 GO:0006386; P:termination of RNA polymerase III transcription; TAS:Reactome.
 GO:0006385; P:transcription elongation from RNA polymerase III promoter; TAS:Reactome. 

Interpro

 IPR007811; RNA_pol_III_Rpc4. 

Pfam

 PF05132; RNA_pol_Rpc4 

SMART

  

PROSITE

  

PRINTS