CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-030966
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Kinesin-like protein KIF20A 
Protein Synonyms/Alias
 cDNA FLJ55710, highly similar to Kinesin family member 20A 
Gene Name
 KIF20A 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
84TLVLQAPKDSFALKSubiquitination[1]
90PKDSFALKSNERGIGubiquitination[1, 2, 3, 4, 5]
132EMVKDVLKGQNWLIYubiquitination[6]
157HTIQGTIKDGGILPRubiquitination[4, 5, 6]
197EVIWLDSKQIRQEEMubiquitination[4, 6]
224EELSTSLKRSVYIESubiquitination[1, 2, 4, 5]
324QNGNPYVKDLNWIHVubiquitination[3]
346KLLKVGRKNQSFASTubiquitination[5]
407QKSGERLKEAGNINTubiquitination[1, 4, 6]
435QNQQNRSKQNLVPFRubiquitination[1, 5]
519QVSPSLEKGAKADTGubiquitination[5]
612EEKLNILKESLTSFYubiquitination[3, 4, 5, 7]
629EIQERDEKIEELEALubiquitination[7]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [7] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931] 
Functional Description
  
Sequence Annotation
  
Keyword
 ATP-binding; Complete proteome; Microtubule; Motor protein; Nucleotide-binding; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 872 AA 
Protein Sequence
MSQGILSPPA GLLSDDDVVV SPMFESTAAD LGSVVRKNLL SDCSVVSTSL EDKQQVPSED 60
SMEKEDQGCV RIENVETLVL QAPKDSFALK SNERGIGQAT HRFTFSQIFG PEVGQASFFN 120
LTVKEMVKDV LKGQNWLIYT YGVTNSGKTH TIQGTIKDGG ILPRSLALIF NSLQGQLHPT 180
PDLKPLLSNE VIWLDSKQIR QEEMKKLSLL NGGLQEEELS TSLKRSVYIE SRIGTSTSFD 240
SGIAGLSSIS QCTSSSQLDE TSHRWAQPDT APLPVPANIR FSIWISFFEI YNELLYDLLE 300
PPSQQRKRQT LRLCEDQNGN PYVKDLNWIH VQDAEEAWKL LKVGRKNQSF ASTHLNQNSS 360
RSHSIFSIRI LHLQGEGDIV PKISELSLCD LAGSERCKDQ KSGERLKEAG NINTSLHTLG 420
RCIAALRQNQ QNRSKQNLVP FRDSKLTRVF QGFFTGRGRS CMIVNVNPCA STYDETLHVA 480
KFSAIASQLV HAPPMQLGFP SLHSFIKEHS LQVSPSLEKG AKADTGLDDD IENEADISMY 540
GKEELLQVVE AMKTLLLKER QEKLQLEMHL RDEICNEMVE QMQQREQWCS EHLDTQKELL 600
EEMYEEKLNI LKESLTSFYQ EEIQERDEKI EELEALLQEA RQQSVAHQQS GSELALRRSQ 660
RLAASASTQQ LQEVKAKLQQ CKAELNSTTE ELHKYQKMLE PPPSAKPFTI DVDKKLEEGQ 720
KNIRLLRTEL QKLGESLQSA ERACCHSTGA GKLRQALTTC DDILIKQDQT LAELQNNMVL 780
VKLDLRKKAA CIAEQYHTVL KLQGQVSAKK RLGTNQENQQ PNQQPPGKKP FLRNLLPRTP 840
TCQSSTDCSP YARILRSRRS PLLKSGPFGK KY 872 
Gene Ontology
 GO:0005794; C:Golgi apparatus; IEA:InterPro.
 GO:0005874; C:microtubule; IEA:UniProtKB-KW.
 GO:0005875; C:microtubule associated complex; IEA:InterPro.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0003777; F:microtubule motor activity; IEA:InterPro.
 GO:0000910; P:cytokinesis; IEA:InterPro.
 GO:0007018; P:microtubule-based movement; IEA:InterPro. 
Interpro
 IPR027326; KIF20A.
 IPR019821; Kinesin_motor_CS.
 IPR001752; Kinesin_motor_dom.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00225; Kinesin 
SMART
 SM00129; KISc 
PROSITE
 PS00411; KINESIN_MOTOR_DOMAIN1
 PS50067; KINESIN_MOTOR_DOMAIN2 
PRINTS
 PR00380; KINESINHEAVY.