UniProt Accession

 H33_PIG; Q71LE2 

Genbank Protein ID

 AF469469 

Genbank Nucleotide ID

 AAL76273.1 

Protein Name

 Histone H3.3 

Protein Synonyms/Alias

  

Gene Name

 H3F3A 

Gene Synonyms/Alias

 H3.3A 

Created Date

 July 27, 2013 

Organism

 Sus scrofa (Pig) 

NCBI Taxa ID

 9823 

Lysine Modification

Position	Peptide	Type	References
10	RTKQTARKSTGGKAP	acetylation	[1]
15	ARKSTGGKAPRKQLA	acetylation	[1, 2]
19	TGGKAPRKQLATKAA	acetylation	[1, 2]
24	PRKQLATKAARKSAP	acetylation	[2]

Reference

 [1] Regulation of chromatin and chromosome morphology by histone H3 modifications in pig oocytes.
 Bui HT, Van Thuan N, Kishigami S, Wakayama S, Hikichi T, Ohta H, Mizutani E, Yamaoka E, Wakayama T, Miyano T.
 Reproduction. 2007 Feb;133(2):371-82. [PMID: 17307905]
 [2] Patterns of histone acetylation.
 Thorne AW, Kmiciek D, Mitchelson K, Sautiere P, Crane-Robinson C.
 Eur J Biochem. 1990 Nov 13;193(3):701-13. [PMID: 2249688] 

Functional Description

 Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. 

Sequence Annotation

 MOD_RES 3 3 Asymmetric dimethylarginine; by PRMT6 (By
 MOD_RES 4 4 Phosphothreonine; by GSG2 (By
 MOD_RES 5 5 Allysine; alternate (By similarity).
 MOD_RES 5 5 N6,N6,N6-trimethyllysine; alternate (By
 MOD_RES 5 5 N6,N6-dimethyllysine; alternate (By
 MOD_RES 5 5 N6-acetyllysine; alternate (By
 MOD_RES 5 5 N6-crotonyl-L-lysine; alternate (By
 MOD_RES 5 5 N6-methyllysine; alternate (By
 MOD_RES 7 7 Phosphothreonine; by PKC (By similarity).
 MOD_RES 9 9 Citrulline; alternate (By similarity).
 MOD_RES 9 9 Symmetric dimethylarginine; by PRMT5;
 MOD_RES 10 10 N6,N6,N6-trimethyllysine; alternate (By
 MOD_RES 10 10 N6,N6-dimethyllysine; alternate (By
 MOD_RES 10 10 N6-acetyllysine; alternate (By
 MOD_RES 10 10 N6-crotonyl-L-lysine; alternate (By
 MOD_RES 10 10 N6-methyllysine; alternate (By
 MOD_RES 11 11 Phosphoserine; by AURKB, AURKC, RPS6KA3,
 MOD_RES 12 12 Phosphothreonine; by PKC (By similarity).
 MOD_RES 15 15 N6-acetyllysine (By similarity).
 MOD_RES 18 18 Asymmetric dimethylarginine; by CARM1;
 MOD_RES 18 18 Citrulline; alternate (By similarity).
 MOD_RES 19 19 N6-acetyllysine; alternate (By
 MOD_RES 19 19 N6-crotonyl-L-lysine; alternate (By
 MOD_RES 19 19 N6-methyllysine; alternate (By
 MOD_RES 24 24 N6-acetyllysine; alternate (By
 MOD_RES 24 24 N6-crotonyl-L-lysine; alternate (By
 MOD_RES 24 24 N6-methyllysine; alternate (By
 MOD_RES 28 28 N6,N6,N6-trimethyllysine; alternate (By
 MOD_RES 28 28 N6,N6-dimethyllysine; alternate (By
 MOD_RES 28 28 N6-acetyllysine; alternate (By
 MOD_RES 28 28 N6-crotonyl-L-lysine; alternate (By
 MOD_RES 28 28 N6-methyllysine; alternate (By
 MOD_RES 29 29 Phosphoserine; by AURKB, AURKC and
 MOD_RES 32 32 Phosphoserine (By similarity).
 MOD_RES 37 37 N6,N6,N6-trimethyllysine; alternate (By
 MOD_RES 37 37 N6,N6-dimethyllysine; alternate (By
 MOD_RES 37 37 N6-acetyllysine; alternate (By
 MOD_RES 37 37 N6-methyllysine; alternate (By
 MOD_RES 38 38 N6-methyllysine (By similarity).
 MOD_RES 42 42 Phosphotyrosine (By similarity).
 MOD_RES 57 57 N6,N6,N6-trimethyllysine; alternate (By
 MOD_RES 57 57 N6-acetyllysine; alternate (By
 MOD_RES 57 57 N6-crotonyl-L-lysine; alternate (By
 MOD_RES 57 57 N6-methyllysine; by EHMT2; alternate (By
 MOD_RES 58 58 Phosphoserine (By similarity).
 MOD_RES 65 65 N6-methyllysine (By similarity).
 MOD_RES 80 80 N6,N6,N6-trimethyllysine; alternate (By
 MOD_RES 80 80 N6,N6-dimethyllysine; alternate (By
 MOD_RES 80 80 N6-acetyllysine; alternate (By
 MOD_RES 80 80 N6-methyllysine; alternate (By
 MOD_RES 81 81 Phosphothreonine (By similarity).
 MOD_RES 108 108 Phosphothreonine (By similarity).
 MOD_RES 123 123 N6-methyllysine (By similarity).  

Keyword

 Acetylation; Chromosome; Citrullination; Complete proteome; DNA-binding; Methylation; Nucleosome core; Nucleus; Phosphoprotein; Reference proteome; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 136 AA 

Protein Sequence

Gene Ontology

 GO:0000786; C:nucleosome; IEA:UniProtKB-KW.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0006334; P:nucleosome assembly; IEA:InterPro. 

Interpro

 IPR009072; Histone-fold.
 IPR007125; Histone_core_D.
 IPR000164; Histone_H3. 

Pfam

 PF00125; Histone 

SMART

 SM00428; H3 

PROSITE

 PS00322; HISTONE_H3_1
 PS00959; HISTONE_H3_2 

PRINTS

 PR00622; HISTONEH3.