CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015955
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 BTB/POZ domain-containing protein KCTD20 
Protein Synonyms/Alias
  
Gene Name
 KCTD20 
Gene Synonyms/Alias
 C6orf69 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
118SHSQAPEKVTLLVDGubiquitination[1, 2, 3, 4, 5, 6]
161NFTRPNEKGEYEIAEubiquitination[2, 4, 7]
183RTVLDYYKTGIINCPubiquitination[4]
297SKLYRFFKYIENRDVubiquitination[4]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [7] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931
Functional Description
  
Sequence Annotation
 DOMAIN 117 191 BTB.  
Keyword
 Alternative splicing; Complete proteome; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 419 AA 
Protein Sequence
MNVHRGSDSD RLLRQEASCL VDDTLAVAQE KEANSLASSG PHNLTYPLGP RNEDLSLDYA 60
SQPANLQFPH IMPLAEDIKG SCFQSGNKRN HEPFIAPERF GNSSVGFGSN SHSQAPEKVT 120
LLVDGTRFVV NPQIFTAHPD TMLGRMFGPG REYNFTRPNE KGEYEIAEGI SATVFRTVLD 180
YYKTGIINCP DGISIPDLRD TCDYLCINFD FNTIRCQDLS ALLHELSNDG AHKQFDHYLE 240
ELILPIMVGC AKKGERECHI VVLTDEDSVD WDEDHPPPMG EEYSQILYSS KLYRFFKYIE 300
NRDVAKTVLK ERGLKNIRIG IEGYPTCKEK IKRRPGGRSE VIYNYVQRPF IQMSWEKEEG 360
KSRHVDFQCV RSKSLTNLVA AGDDVLEDQE ILMHHPPQVD ELDRLNAPLS QMASNDFQD 419 
Gene Ontology
 GO:0051260; P:protein homooligomerization; IEA:InterPro. 
Interpro
 IPR000210; BTB/POZ-like.
 IPR011333; BTB/POZ_fold.
 IPR003131; T1-type_BTB. 
Pfam
 PF02214; K_tetra 
SMART
 SM00225; BTB 
PROSITE
 PS50097; BTB 
PRINTS