CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019233
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 E3 ubiquitin-protein ligase pellino homolog 1 
Protein Synonyms/Alias
 Pellino-1; Pellino-related intracellular-signaling molecule 
Gene Name
 PELI1 
Gene Synonyms/Alias
 PRISM 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
12DQENHPSKAPVKYGEubiquitination[1]
16HPSKAPVKYGELIVLubiquitination[1]
162AAGFDSSKNIFLGEKubiquitination[1]
169KNIFLGEKAAKWKTSubiquitination[1]
202NGFTEDSKPGIWREIsumoylation[2]
202NGFTEDSKPGIWREIubiquitination[1]
266LSHTPTVKHLEALRQsumoylation[2]
266LSHTPTVKHLEALRQubiquitination[1, 3, 4]
295TLAFPSMKRKDVVDEsumoylation[2]
297AFPSMKRKDVVDEKQsumoylation[2]
303RKDVVDEKQPWVYLNsumoylation[2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Pellino-1, an adaptor protein of interleukin-1 receptor/toll-like receptor signaling, is sumoylated by Ubc9.
 Kim JH, Sung KS, Jung SM, Lee YS, Kwon JY, Choi CY, Park SH.
 Mol Cells. 2011 Jan;31(1):85-9. [PMID: 21120624]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6. Mediates 'Lys-63'-linked polyubiquitination of IRAK1 allowing subsequent NF-kappa-B activation. 
Sequence Annotation
  
Keyword
 Complete proteome; Ligase; Phosphoprotein; Reference proteome; Ubl conjugation; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 418 AA 
Protein Sequence
MFSPDQENHP SKAPVKYGEL IVLGYNGSLP NGDRGRRKSR FALFKRPKAN GVKPSTVHIA 60
CTPQAAKAIS NKDQHSISYT LSRAQTVVVE YTHDSNTDMF QIGRSTESPI DFVVTDTVPG 120
SQSNSDTQSV QSTISRFACR IICERNPPFT ARIYAAGFDS SKNIFLGEKA AKWKTSDGQM 180
DGLTTNGVLV MHPRNGFTED SKPGIWREIS VCGNVFSLRE TRSAQQRGKM VEIETNQLQD 240
GSLIDLCGAT LLWRTAEGLS HTPTVKHLEA LRQEINAARP QCPVGFNTLA FPSMKRKDVV 300
DEKQPWVYLN CGHVHGYHNW GNKEERDGKD RECPMCRSVG PYVPLWLGCE AGFYVDAGPP 360
THAFSPCGHV CSEKTTAYWS QIPLPHGTHT FHAACPFCAH QLAGEQGYIR LIFQGPLD 418 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005634; C:nucleus; IEA:Compara.
 GO:0004842; F:ubiquitin-protein ligase activity; IEA:Compara.
 GO:0045087; P:innate immune response; TAS:Reactome.
 GO:0002755; P:MyD88-dependent toll-like receptor signaling pathway; TAS:Reactome.
 GO:0032088; P:negative regulation of NF-kappaB transcription factor activity; IEA:Compara.
 GO:0042130; P:negative regulation of T cell proliferation; IEA:Compara.
 GO:0030890; P:positive regulation of B cell proliferation; IEA:Compara.
 GO:0001819; P:positive regulation of cytokine production; IEA:Compara.
 GO:0043123; P:positive regulation of I-kappaB kinase/NF-kappaB cascade; TAS:Reactome.
 GO:0031398; P:positive regulation of protein ubiquitination; IEA:Compara.
 GO:0034141; P:positive regulation of toll-like receptor 3 signaling pathway; IEA:Compara.
 GO:0034145; P:positive regulation of toll-like receptor 4 signaling pathway; IEA:Compara.
 GO:0070936; P:protein K48-linked ubiquitination; IEA:Compara.
 GO:0043331; P:response to dsRNA; IEA:Compara.
 GO:0032496; P:response to lipopolysaccharide; IEA:Compara.
 GO:0008063; P:Toll signaling pathway; IEA:InterPro.
 GO:0034166; P:toll-like receptor 10 signaling pathway; TAS:Reactome.
 GO:0034134; P:toll-like receptor 2 signaling pathway; TAS:Reactome.
 GO:0034142; P:toll-like receptor 4 signaling pathway; TAS:Reactome.
 GO:0034146; P:toll-like receptor 5 signaling pathway; TAS:Reactome.
 GO:0034162; P:toll-like receptor 9 signaling pathway; TAS:Reactome.
 GO:0038123; P:toll-like receptor TLR1:TLR2 signaling pathway; TAS:Reactome.
 GO:0038124; P:toll-like receptor TLR6:TLR2 signaling pathway; TAS:Reactome. 
Interpro
 IPR006800; Pellino_fam. 
Pfam
 PF04710; Pellino 
SMART
  
PROSITE
  
PRINTS