UniProt Accession

 NFI_ECOLI; P68739; P32679; Q2M8T5 

Genbank Protein ID

 U00006; U00096; AP009048 

Genbank Nucleotide ID

 AAC43096.1; AAC76972.2; BAE77321.1 

Protein Name

 Endonuclease V 

Protein Synonyms/Alias

 Deoxyinosine 3'endonuclease; Deoxyribonuclease V; DNase V 

Gene Name

 nfi 

Gene Synonyms/Alias

 yjaF; b3998; JW5547 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
53	RAAMVLLKYPSLELV	acetylation	[1]
63	SLELVEYKVARIATT	acetylation	[1]
138	AKKRLCGKFEPLSSE	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. Has a wide substrate spectrum. In addition to deoxyinosine-containing DNA, the enzyme cleaves DNA containing urea residues, AP sites, base mismatches, insertion/deletion mismatches, flaps, and pseudo-Y structures. Participates in the excision repair of hypoxanthine and xanthine (deaminated adenine and guanine) in DNA. It thereby reduces the mutagenic effects of nitrous acid by attacking lesions caused by nitrosative deamination. 

Sequence Annotation

 METAL 35 35 Magnesium (By similarity).
 METAL 103 103 Magnesium (By similarity).  

Keyword

 Complete proteome; Cytoplasm; Direct protein sequencing; DNA damage; DNA repair; Endonuclease; Hydrolase; Magnesium; Metal-binding; Nuclease; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 223 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0043737; F:deoxyribonuclease V activity; IDA:EcoCyc.
 GO:0000287; F:magnesium ion binding; IEA:HAMAP.
 GO:0006281; P:DNA repair; IEA:HAMAP. 

Interpro

 IPR007581; Endonuclease-V. 

Pfam

 PF04493; Endonuclease_5 

SMART

  

PROSITE

  

PRINTS