CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014619
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Steroid 17-alpha-hydroxylase/17,20 lyase 
Protein Synonyms/Alias
 17-alpha-hydroxyprogesterone aldolase; CYPXVII; Cytochrome P450 17A1; Cytochrome P450-C17; Cytochrome P450c17 
Gene Name
 CYP17A1 
Gene Synonyms/Alias
 CYP17 
Created Date
 July 27, 2013 
Organism
 Cavia porcellus (Guinea pig) 
NCBI Taxa ID
 10141 
Lysine Modification
Position
Peptide
Type
References
29SSGTKYPKSLPSLPVacetylation[1]
58VNFFKLQKKYGPIYSacetylation[2]
59NFFKLQKKYGPIYSFacetylation[1, 2]
91ELLIKKGKEFSGRPLacetylation[1, 2]
222VDIFPWLKIFPNKTLacetylation[1]
227WLKIFPNKTLEMIRKacetylation[1, 2]
234KTLEMIRKYTEIRGAacetylation[1, 2]
326LHNPQVKKKIQEEIDacetylation[2]
327HNPQVKKKIQEEIDHacetylation[1, 2]
374APILIPHKANTDSSIacetylation[1]
388IGEFAIDKDTNVLVNacetylation[1]
490VFLIDPFKVKITVRPacetylation[1]
492LIDPFKVKITVRPAWacetylation[1]
Reference
 [1] Membrane topology of guinea pig cytochrome P450 17 alpha revealed by a combination of chemical modifications and mass spectrometry.
 Izumi S, Kaneko H, Yamazaki T, Hirata T, Kominami S.
 Biochemistry. 2003 Dec 16;42(49):14663-9. [PMID: 14661979]
 [2] The interaction of cytochrome P450 17alpha with NADPH-cytochrome P450 reductase, investigated using chemical modification and MALDI-TOF mass spectrometry.
 Nikfarjam L, Izumi S, Yamazaki T, Kominami S.
 Biochim Biophys Acta. 2006 Jun;1764(6):1126-31. [PMID: 16713412] 
Functional Description
 Conversion of pregnenolone and progesterone to their 17- alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty. 
Sequence Annotation
 METAL 442 442 Iron (heme axial ligand) (By similarity).  
Keyword
 Complete proteome; Direct protein sequencing; Heme; Iron; Lyase; Membrane; Metal-binding; Monooxygenase; Oxidoreductase; Reference proteome; Steroidogenesis. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 508 AA 
Protein Sequence
MWELVTLLGL ILAYLFWPRQ GSSGTKYPKS LPSLPVVGSL PFLPKSGHMH VNFFKLQKKY 60
GPIYSFRLGS TTTVVIGHHQ LARELLIKKG KEFSGRPLTT TVALLSDNGK GIAFADSSAT 120
WQLHRRLVLS SFSLFRDGEQ KLENIICQEL SALCDFLATC DGQVKDLSSS IFMTVVNIIC 180
MICFSVSYKE GDMELVTIRR FTTGFVNSLS DDNLVDIFPW LKIFPNKTLE MIRKYTEIRG 240
AMLSKILKEC KEKFRSDSVS NLIDLLIQAK VNENNNNSSL DQDSNLFSDK HILTTLGDIF 300
GAGVETSSSV VLWVIAFLLH NPQVKKKIQE EIDHNVGFSR TPTFSDRNHL LMLEATIREV 360
LRIRPVAPIL IPHKANTDSS IGEFAIDKDT NVLVNLWALH HNEQEWDRPD QFMPERFLDP 420
TGSQIIVPSS SYLPFGAGPR SCVGEALARQ EIFLITAWLL QKFDLEVPEG GQLPSLEGIP 480
KIVFLIDPFK VKITVRPAWK EAQAEGSA 508 
Gene Ontology
 GO:0030424; C:axon; IEA:Compara.
 GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0043025; C:neuronal cell body; IEA:Compara.
 GO:0047442; F:17-alpha-hydroxyprogesterone aldolase activity; IEA:EC.
 GO:0009055; F:electron carrier activity; IEA:InterPro.
 GO:0020037; F:heme binding; ISS:UniProtKB.
 GO:0005506; F:iron ion binding; IEA:InterPro.
 GO:0004508; F:steroid 17-alpha-monooxygenase activity; ISS:UniProtKB.
 GO:0006704; P:glucocorticoid biosynthetic process; IEA:Compara.
 GO:0042446; P:hormone biosynthetic process; ISS:UniProtKB.
 GO:0042448; P:progesterone metabolic process; ISS:UniProtKB. 
Interpro
 IPR001128; Cyt_P450.
 IPR017972; Cyt_P450_CS.
 IPR002401; Cyt_P450_E_grp-I. 
Pfam
 PF00067; p450 
SMART
  
PROSITE
 PS00086; CYTOCHROME_P450 
PRINTS
 PR00463; EP450I.
 PR00385; P450.