CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004367
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Heat shock protein STI1 
Protein Synonyms/Alias
  
Gene Name
 STI1 
Gene Synonyms/Alias
 YOR027W; OR26.17 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
23FTAKDYDKAIELFTKacetylation[1]
54SACYTSLKKFSDALNubiquitination[2]
68NDANECVKINPSWSKubiquitination[2]
75KINPSWSKGYNRLGAacetylation[1]
75KINPSWSKGYNRLGAubiquitination[2]
98DEAESNYKKALELDAacetylation[1]
99EAESNYKKALELDASubiquitination[2, 3]
108LELDASNKAAKEGLDubiquitination[2]
150ENLKKNPKTSEMMKDubiquitination[2]
156PKTSEMMKDPQLVAKubiquitination[2, 3]
163KDPQLVAKLIGYKQNacetylation[1]
168VAKLIGYKQNPQAIGubiquitination[2, 3]
233QSDSTTSKENSSKAPubiquitination[2]
259EVDEDDSKIEADKEKacetylation[1]
308RAAAEYEKGEYETAIubiquitination[2]
361KTIEYYQKSLTEHRTacetylation[1]
374RTADILTKLRNAEKEacetylation[1]
384NAEKELKKAEAEAYVubiquitination[3]
395EAYVNPEKAEEARLEubiquitination[2]
404EEARLEGKEYFTKSDacetylation[1]
404EEARLEGKEYFTKSDubiquitination[2]
409EGKEYFTKSDWPNAVubiquitination[2]
442NRAAALAKLMSFPEAacetylation[1]
459DCNKAIEKDPNFVRAacetylation[1]
493TLDAARTKDAEVNNGubiquitination[2]
512EIDQLYYKASQQRFQacetylation[1]
512EIDQLYYKASQQRFQubiquitination[2]
574MKNPEVFKKIQTLIAubiquitination[2]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [3] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 
Functional Description
 May play a role in mediating the heat shock response of some HSP70 genes. It is required for optimal growth of yeast cells at both low and high temperature. 
Sequence Annotation
 REPEAT 5 38 TPR 1.
 REPEAT 40 73 TPR 2.
 REPEAT 74 107 TPR 3.
 DOMAIN 138 177 STI1 1.
 REPEAT 262 295 TPR 4.
 REPEAT 297 328 TPR 5.
 REPEAT 336 369 TPR 6.
 REPEAT 396 429 TPR 7.
 REPEAT 430 463 TPR 8.
 REPEAT 465 492 TPR 9.
 DOMAIN 537 576 STI1 2.
 MOD_RES 227 227 Phosphoserine.  
Keyword
 3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; Glycoprotein; Phosphoprotein; Reference proteome; Repeat; Stress response; TPR repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 589 AA 
Protein Sequence
MSLTADEYKQ QGNAAFTAKD YDKAIELFTK AIEVSETPNH VLYSNRSACY TSLKKFSDAL 60
NDANECVKIN PSWSKGYNRL GAAHLGLGDL DEAESNYKKA LELDASNKAA KEGLDQVHRT 120
QQARQAQPDL GLTQLFADPN LIENLKKNPK TSEMMKDPQL VAKLIGYKQN PQAIGQDLFT 180
DPRLMTIMAT LMGVDLNMDD INQSNSMPKE PETSKSTEQK KDAEPQSDST TSKENSSKAP 240
QKEESKESEP MEVDEDDSKI EADKEKAEGN KFYKARQFDE AIEHYNKAWE LHKDITYLNN 300
RAAAEYEKGE YETAISTLND AVEQGREMRA DYKVISKSFA RIGNAYHKLG DLKKTIEYYQ 360
KSLTEHRTAD ILTKLRNAEK ELKKAEAEAY VNPEKAEEAR LEGKEYFTKS DWPNAVKAYT 420
EMIKRAPEDA RGYSNRAAAL AKLMSFPEAI ADCNKAIEKD PNFVRAYIRK ATAQIAVKEY 480
ASALETLDAA RTKDAEVNNG SSAREIDQLY YKASQQRFQP GTSNETPEET YQRAMKDPEV 540
AAIMQDPVMQ SILQQAQQNP AALQEHMKNP EVFKKIQTLI AAGIIRTGR 589 
Gene Ontology
 GO:0005737; C:cytoplasm; IPI:SGD.
 GO:0042030; F:ATPase inhibitor activity; IDA:SGD.
 GO:0030544; F:Hsp70 protein binding; IDA:SGD.
 GO:0051879; F:Hsp90 protein binding; IDA:SGD.
 GO:0003729; F:mRNA binding; IDA:SGD.
 GO:0006457; P:protein folding; IMP:SGD.
 GO:0006950; P:response to stress; IEA:UniProtKB-KW. 
Interpro
 IPR006636; STI1_HS-bd.
 IPR001440; TPR-1.
 IPR013026; TPR-contain_dom.
 IPR011990; TPR-like_helical.
 IPR019734; TPR_repeat. 
Pfam
 PF00515; TPR_1 
SMART
 SM00727; STI1
 SM00028; TPR 
PROSITE
 PS50005; TPR
 PS50293; TPR_REGION 
PRINTS