CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005077
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Replication factor A protein 1 
Protein Synonyms/Alias
 RF-A protein 1; DNA-binding protein BUF2; Replication protein A 69 kDa DNA-binding subunit; Single-stranded DNA-binding protein 
Gene Name
 RFA1 
Gene Synonyms/Alias
 BUF2; RPA1; YAR007C; FUN3 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
427NANFITLKQEPGMGGacetylation[1]
463ENLGRSEKGDFFSVKacetylation[1]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919
Functional Description
 Binds to single-stranded sequences participating in DNA replication in addition to those mediating transcriptional repression (URS1) and activation (CAR1). Stimulates the activity of a cognate strand exchange protein (SEP1). It cooperates with T- AG and DNA topoisomerase I to unwind template DNA containing the simian virus 40 origin of DNA replication. 
Sequence Annotation
 ZN_FING 486 508 C4-type (Potential).
 MOD_RES 178 178 Phosphoserine; by ATM or ATR.  
Keyword
 3D-structure; Complete proteome; Direct protein sequencing; DNA replication; DNA-binding; Metal-binding; Nucleus; Phosphoprotein; Reference proteome; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 621 AA 
Protein Sequence
MSSVQLSRGD FHSIFTNKQR YDNPTGGVYQ VYNTRKSDGA NSNRKNLIMI SDGIYHMKAL 60
LRNQAASKFQ SMELQRGDII RVIIAEPAIV RERKKYVLLV DDFELVQSRA DMVNQTSTFL 120
DNYFSEHPNE TLKDEDITDS GNVANQTNAS NAGVPDMLHS NSNLNANERK FANENPNSQK 180
TRPIFAIEQL SPYQNVWTIK ARVSYKGEIK TWHNQRGDGK LFNVNFLDTS GEIRATAFND 240
FATKFNEILQ EGKVYYVSKA KLQPAKPQFT NLTHPYELNL DRDTVIEECF DESNVPKTHF 300
NFIKLDAIQN QEVNSNVDVL GIIQTINPHF ELTSRAGKKF DRRDITIVDD SGFSISVGLW 360
NQQALDFNLP EGSVAAIKGV RVTDFGGKSL SMGFSSTLIP NPEIPEAYAL KGWYDSKGRN 420
ANFITLKQEP GMGGQSAASL TKFIAQRITI ARAQAENLGR SEKGDFFSVK AAISFLKVDN 480
FAYPACSNEN CNKKVLEQPD GTWRCEKCDT NNARPNWRYI LTISIIDETN QLWLTLFDDQ 540
AKQLLGVDAN TLMSLKEEDP NEFTKITQSI QMNEYDFRIR AREDTYNDQS RIRYTVANLH 600
SLNYRAEADY LADELSKALL A 621 
Gene Ontology
 GO:0000781; C:chromosome, telomeric region; IMP:SGD.
 GO:0000794; C:condensed nuclear chromosome; IDA:SGD.
 GO:0005829; C:cytosol; IDA:SGD.
 GO:0005662; C:DNA replication factor A complex; IDA:SGD.
 GO:0003690; F:double-stranded DNA binding; IDA:SGD.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0043565; F:sequence-specific DNA binding; IDA:SGD.
 GO:0003697; F:single-stranded DNA binding; IDA:SGD.
 GO:0006268; P:DNA unwinding involved in replication; IDA:SGD.
 GO:0000724; P:double-strand break repair via homologous recombination; IGI:SGD.
 GO:0045184; P:establishment of protein localization; IPI:SGD.
 GO:0030491; P:heteroduplex formation; IDA:SGD.
 GO:0006289; P:nucleotide-excision repair; IDA:SGD.
 GO:0016567; P:protein ubiquitination; IMP:SGD.
 GO:0007131; P:reciprocal meiotic recombination; IMP:SGD.
 GO:0000722; P:telomere maintenance via recombination; IGI:SGD.
 GO:0007004; P:telomere maintenance via telomerase; IPI:SGD. 
Interpro
 IPR012340; NA-bd_OB-fold.
 IPR004365; NA-bd_OB_tRNA-helicase.
 IPR013955; Rep_factor-A_C.
 IPR007199; Rep_factor-A_N.
 IPR004591; Rep_factor_Rpa1. 
Pfam
 PF04057; Rep-A_N
 PF08646; Rep_fac-A_C
 PF01336; tRNA_anti 
SMART
  
PROSITE
  
PRINTS