CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-009752
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform 
Protein Synonyms/Alias
 PP2A-alpha 
Gene Name
 Ppp2ca 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
4****MDEKLFTKELDacetylation[1]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 
Functional Description
 PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I. Activates RAF1 by dephosphorylating it at 'Ser-259' (By similarity). 
Sequence Annotation
 ACT_SITE 118 118 Proton donor (By similarity).
 METAL 57 57 Iron (By similarity).
 METAL 59 59 Iron (By similarity).
 METAL 85 85 Iron (By similarity).
 METAL 85 85 Manganese (By similarity).
 METAL 117 117 Manganese (By similarity).
 METAL 167 167 Manganese (By similarity).
 METAL 241 241 Manganese (By similarity).
 MOD_RES 4 4 N6-acetyllysine (By similarity).
 MOD_RES 307 307 Phosphotyrosine (By similarity).
 MOD_RES 309 309 Leucine methyl ester (By similarity).  
Keyword
 Acetylation; Centromere; Chromosome; Complete proteome; Cytoplasm; Cytoskeleton; Hydrolase; Iron; Manganese; Meiosis; Metal-binding; Methylation; Nucleus; Phosphoprotein; Protein phosphatase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 309 AA 
Protein Sequence
MDEKLFTKEL DQWIEQLNEC KQLSESQVKS LCEKAKEILT KESNVQEVRC PVTVCGDVHG 60
QFHDLMELFR IGGKSPDTNY LFMGDYVDRG YYSVETVTLL VALKVRYRER ITILRGNHES 120
RQITQVYGFY DECLRKYGNA NVWKYFTDLF DYLPLTALVD GQIFCLHGGL SPSIDTLDHI 180
RALDRLQEVP HEGPMCDLLW SDPDDRGGWG ISPRGAGYTF GQDISETFNH ANGLTLVSRA 240
HQLVMEGYNW CHDRNVVTIF SAPNYCYRCG NQAAIMELDD TLKYSFLQFD PAPRRGEPHV 300
TRRTPDYFL 309 
Gene Ontology
 GO:0000775; C:chromosome, centromeric region; IEA:UniProtKB-SubCell.
 GO:0005829; C:cytosol; IEA:Compara.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; IEA:Compara.
 GO:0000159; C:protein phosphatase type 2A complex; IEA:Compara.
 GO:0000922; C:spindle pole; IEA:UniProtKB-SubCell.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0046982; F:protein heterodimerization activity; ISS:UniProtKB.
 GO:0004722; F:protein serine/threonine phosphatase activity; IDA:RGD.
 GO:0007126; P:meiosis; IEA:UniProtKB-KW.
 GO:0007498; P:mesoderm development; IEA:Compara.
 GO:0010719; P:negative regulation of epithelial to mesenchymal transition; IEA:Compara.
 GO:0071902; P:positive regulation of protein serine/threonine kinase activity; IEA:Compara.
 GO:0006470; P:protein dephosphorylation; IDA:RGD.
 GO:0070208; P:protein heterotrimerization; IMP:MGI.
 GO:0031952; P:regulation of protein autophosphorylation; IMP:MGI.
 GO:0042176; P:regulation of protein catabolic process; IMP:MGI.
 GO:0010469; P:regulation of receptor activity; IMP:MGI. 
Interpro
 IPR004843; Metallo_PEstase_dom.
 IPR006186; Ser/Thr-sp_prot-phosphatase. 
Pfam
 PF00149; Metallophos 
SMART
 SM00156; PP2Ac 
PROSITE
 PS00125; SER_THR_PHOSPHATASE 
PRINTS
 PR00114; STPHPHTASE.