CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012969
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Inactive hydroxysteroid dehydrogenase-like protein 1 
Protein Synonyms/Alias
  
Gene Name
 HSDL1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
64GSRADLIKQYGRWAVubiquitination[1, 2, 3]
81GATDGIGKAYAEELAubiquitination[1, 2, 3]
103LISRNEEKLQVVAKDubiquitination[1, 2, 3]
109EKLQVVAKDIADTYKubiquitination[1, 3, 4, 5, 6]
143IREALKDKDVGILVNubiquitination[1]
287VSTLGISKRTTGYWSubiquitination[2]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
  
Sequence Annotation
 NP_BIND 74 80 NADP (By similarity).
 REGION 2 82 Required for mitochondria translocation.
 BINDING 125 125 NADP (By similarity).
 BINDING 222 222 NADP (By similarity).
 MOD_RES 2 2 N-acetylalanine.  
Keyword
 Acetylation; Alternative splicing; Complete proteome; Direct protein sequencing; Mitochondrion; NADP; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 330 AA 
Protein Sequence
MAAVDSFYLL YREIARSCNC YMEALALVGA WYTARKSITV ICDFYSLIRL HFIPRLGSRA 60
DLIKQYGRWA VVSGATDGIG KAYAEELASR GLNIILISRN EEKLQVVAKD IADTYKVETD 120
IIVADFSSGR EIYLPIREAL KDKDVGILVN NVGVFYPYPQ YFTQLSEDKL WDIINVNIAA 180
ASLMVHVVLP GMVERKKGAI VTISSGSCCK PTPQLAAFSA SKAYLDHFSR ALQYEYASKG 240
IFVQSLIPFY VATSMTAPSN FLHRCSWLVP SPKVYAHHAV STLGISKRTT GYWSHSIQFL 300
FAQYMPEWLW VWGANILNRS LRKEALSCTA 330 
Gene Ontology
 GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
 GO:0016491; F:oxidoreductase activity; IEA:InterPro.
 GO:0055114; P:oxidation-reduction process; IEA:GOC. 
Interpro
 IPR002198; DH_sc/Rdtase_SDR.
 IPR002347; Glc/ribitol_DH.
 IPR016040; NAD(P)-bd_dom. 
Pfam
 PF00106; adh_short 
SMART
  
PROSITE
 PS00061; ADH_SHORT 
PRINTS
 PR00081; GDHRDH.
 PR00080; SDRFAMILY.