CPLM-012069

Genbank Nucleotide ID

Centromere protein R

Protein Synonyms/Alias

CENP-R; Beta-3-endonexin; Integrin beta-3-binding protein; Nuclear receptor-interacting factor 3

Homo sapiens (Human)

Position	Peptide	Type	References
76	HPSLTESKESTTKDN	ubiquitination	[1]
81	ESKESTTKDNDEFMM	ubiquitination	[1]
92	EFMMLLSKVEKLSEE	ubiquitination	[1]
133	SCASHFLKREMQKTK	ubiquitination	[2, 3]
154	NKQKLFEKSTGLPHK	ubiquitination	[4]
173	LDSYEFLKAILN***	ubiquitination	[1]

[1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[2] Global identification of modular cullin-RING ligase substrates.
Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
[3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]

Functional Description

Transcription coregulator that can have both coactivator and corepressor functions. Isoform 1, but not other isoforms, is involved in the coactivation of nuclear receptors for retinoid X (RXRs) and thyroid hormone (TRs) in a ligand-dependent fashion. In contrast, it does not coactivate nuclear receptors for retinoic acid, vitamin D, progesterone receptor, nor glucocorticoid. Acts as a coactivator for estrogen receptor alpha. Acts as a transcriptional corepressor via its interaction with the NFKB1 NF- kappa-B subunit, possibly by interfering with the transactivation domain of NFKB1. Induces apoptosis in breast cancer cells, but not in other cancer cells, via a caspase-2 mediated pathway that involves mitochondrial membrane permeabilization but does not require other caspases. May also act as an inhibitor of cyclin A- associated kinase. Also acts a component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex.

REGION 20 50 DD1.
MOTIF 9 13 LXXLL motif.
MOTIF 63 66 Nuclear localization signal.
MOTIF 172 176 LXXIL motif.
MOD_RES 17 17 Phosphoserine.
MOD_RES 28 28 Phosphoserine (Probable).
MOD_RES 71 71 Phosphoserine.

Activator; Alternative splicing; Apoptosis; Cell cycle; Cell division; Centromere; Chromosome; Coiled coil; Complete proteome; Cytoplasm; Kinetochore; Mitosis; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repressor; Transcription; Transcription regulation.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0000777; C:condensed chromosome kinetochore; IEA:UniProtKB-SubCell.
GO:0005829; C:cytosol; TAS:Reactome.
GO:0016020; C:membrane; TAS:ProtInc.
GO:0005654; C:nucleoplasm; TAS:Reactome.
GO:0008022; F:protein C-terminus binding; TAS:ProtInc.
GO:0004871; F:signal transducer activity; TAS:ProtInc.
GO:0097190; P:apoptotic signaling pathway; TAS:Reactome.
GO:0007155; P:cell adhesion; TAS:ProtInc.
GO:0051301; P:cell division; IEA:UniProtKB-KW.
GO:0034080; P:CENP-A containing nucleosome assembly at centromere; TAS:Reactome.
GO:0007067; P:mitosis; IEA:UniProtKB-KW.
GO:0000278; P:mitotic cell cycle; TAS:Reactome.
GO:0048011; P:neurotrophin TRK receptor signaling pathway; TAS:Reactome.
GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.

IPR009601; NRIF3_coact_rcpt.