UniProt Accession

 YBAK_ECOLI; P0AAR3; P37175; P77281; Q2MBU6 

Genbank Protein ID

 U82664; U00096; AP009048; X03895 

Genbank Nucleotide ID

 AAB40235.1; AAC73583.1; BAE76260.1 

Protein Name

 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK 

Protein Synonyms/Alias

  

Gene Name

 ybaK 

Gene Synonyms/Alias

 b0481; JW0470 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
6	**MTPAVKLLEKNKI	acetylation	[1]
10	PAVKLLEKNKISFQI	acetylation	[1]
12	VKLLEKNKISFQIHT	acetylation	[1]
35	NFGDEVVKKLGLNPD	acetylation	[1]
46	LNPDQVYKTLLVAVN	acetylation	[1]
73	VAGQLDLKKVAKALG	acetylation	[1]
74	AGQLDLKKVAKALGA	acetylation	[1]
82	VAKALGAKKVEMADP	acetylation	[1]
83	AKALGAKKVEMADPM	acetylation	[1]
109	GISPLGQKKRLPTII	acetylation	[1]
110	ISPLGQKKRLPTIID	acetylation	[1]
132	TIYVSGGKRGLDIEL	acetylation	[1]
146	LAAGDLAKILDAKFA	acetylation	[1]
151	LAKILDAKFADIARR	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Functions in trans to edit the amino acid from incorrectly charged Cys-tRNA(Pro) via a Cys-tRNA(Pro) deacylase activity. May compensate for the lack of Cys-tRNA(Pro) editing by ProRS. Is also able to deacylate Cys-tRNA(Cys), and displays weak deacylase activity in vitro against Gly-tRNA(Gly), as well as, at higher concentrations, some other correctly charged tRNAs. Unlike some of its orthologs it is not able to remove the amino acid moiety from incorrectly charged Ala-tRNA(Pro). 

Sequence Annotation

  

Keyword

 3D-structure; Complete proteome; Cytoplasm; Lyase; Protein biosynthesis; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 159 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0043907; F:Cys-tRNA(Pro) hydrolase activity; IDA:EcoCyc.
 GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
 GO:0006412; P:translation; IEA:UniProtKB-KW. 

Interpro

 IPR004369; Prolyl-tRNA_editing_YbaK/EbsC.
 IPR007214; YbaK/aa-tRNA-synth-assoc-dom. 

Pfam

 PF04073; YbaK 

SMART

  

PROSITE

  

PRINTS