CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002296
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Beta-glucuronidase 
Protein Synonyms/Alias
 GUS; Beta-D-glucuronoside glucuronosohydrolase 
Gene Name
 uidA 
Gene Synonyms/Alias
 gurA; gusA; b1617; JW1609 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
277GIRSVAVKGEQFLINacetylation[1, 2]
286EQFLINHKPFYFTGFacetylation[2]
487TAEKVLEKELLAWQEacetylation[1, 2]
586SAAFLLQKRWTGMNFacetylation[1, 2]
Reference
 [1] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111]
 [2] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
  
Sequence Annotation
 ACT_SITE 413 413 Proton donor (By similarity).  
Keyword
 3D-structure; Complete proteome; Direct protein sequencing; Glycosidase; Hydrolase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 603 AA 
Protein Sequence
MLRPVETPTR EIKKLDGLWA FSLDRENCGI DQRWWESALQ ESRAIAVPGS FNDQFADADI 60
RNYAGNVWYQ REVFIPKGWA GQRIVLRFDA VTHYGKVWVN NQEVMEHQGG YTPFEADVTP 120
YVIAGKSVRI TVCVNNELNW QTIPPGMVIT DENGKKKQSY FHDFFNYAGI HRSVMLYTTP 180
NTWVDDITVV THVAQDCNHA SVDWQVVANG DVSVELRDAD QQVVATGQGT SGTLQVVNPH 240
LWQPGEGYLY ELCVTAKSQT ECDIYPLRVG IRSVAVKGEQ FLINHKPFYF TGFGRHEDAD 300
LRGKGFDNVL MVHDHALMDW IGANSYRTSH YPYAEEMLDW ADEHGIVVID ETAAVGFNLS 360
LGIGFEAGNK PKELYSEEAV NGETQQAHLQ AIKELIARDK NHPSVVMWSI ANEPDTRPQG 420
AREYFAPLAE ATRKLDPTRP ITCVNVMFCD AHTDTISDLF DVLCLNRYYG WYVQSGDLET 480
AEKVLEKELL AWQEKLHQPI IITEYGVDTL AGLHSMYTDM WSEEYQCAWL DMYHRVFDRV 540
SAVVGEQVWN FADFATSQGI LRVGGNKKGI FTRDRKPKSA AFLLQKRWTG MNFGEKPQQG 600
GKQ 603 
Gene Ontology
 GO:0004566; F:beta-glucuronidase activity; IDA:EcoCyc.
 GO:0019391; P:glucuronoside catabolic process; IMP:EcoCyc. 
Interpro
 IPR008979; Galactose-bd-like.
 IPR006101; Glyco_hydro_2.
 IPR013812; Glyco_hydro_2/20_Ig-like.
 IPR023232; Glyco_hydro_2_AS.
 IPR023230; Glyco_hydro_2_CS.
 IPR006102; Glyco_hydro_2_Ig-like.
 IPR006104; Glyco_hydro_2_N.
 IPR006103; Glyco_hydro_2_TIM.
 IPR013781; Glyco_hydro_catalytic_dom.
 IPR017853; Glycoside_hydrolase_SF. 
Pfam
 PF00703; Glyco_hydro_2
 PF02836; Glyco_hydro_2_C
 PF02837; Glyco_hydro_2_N 
SMART
  
PROSITE
 PS00719; GLYCOSYL_HYDROL_F2_1
 PS00608; GLYCOSYL_HYDROL_F2_2 
PRINTS
 PR00132; GLHYDRLASE2.