CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-030618
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 SUMO-activating enzyme subunit 2 
Protein Synonyms/Alias
 cDNA FLJ42740 fis, clone BRAWH2016655, highly similar to Ubiquitin-like 1-activating enzyme E1B 
Gene Name
 UBA2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
57GQVTTIKKGVTECYEubiquitination[1, 2]
68ECYECHPKPTQRTFPubiquitination[1, 2]
145DIKRISTKEWAKSTGubiquitination[1, 3]
149ISTKEWAKSTGYDPVubiquitination[1, 2, 3]
157STGYDPVKLFTKLFKubiquitination[3]
161DPVKLFTKLFKDDIRubiquitination[1, 3]
164KLFTKLFKDDIRYLLubiquitination[1]
175RYLLTMDKLWRKRKPacetylation[4, 5]
175RYLLTMDKLWRKRKPmethylation[6]
175RYLLTMDKLWRKRKPubiquitination[1, 3]
220DQQVLDVKSYARLFSubiquitination[1, 3, 7]
228SYARLFSKSIETLRVubiquitination[1, 3]
240LRVHLAEKGDGAELIubiquitination[3]
313GLKILSGKIDQCRTIubiquitination[2]
324CRTIFLNKQPNPRKKubiquitination[1, 2, 3]
371TVLTLQDKIVKEKFAubiquitination[1, 3]
376QDKIVKEKFAMVAPDubiquitination[3]
409TEANNHKKLSEFGIRubiquitination[1]
521RKRKLDEKENLSAKRacetylation[2, 4]
527EKENLSAKRSRIEQKacetylation[2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [5] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [6] Large-scale global identification of protein lysine methylation in vivo.
 Cao XJ, Arnaudo AM, Garcia BA.
 Epigenetics. 2013 May 1;8(5):477-85. [PMID: 23644510]
 [7] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
  
Sequence Annotation
  
Keyword
 ATP-binding; Complete proteome; Ligase; Nucleotide-binding; Reference proteome; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 544 AA 
Protein Sequence
MNPDYNVEFF RQFILVMNAL DNRAARNHVN RMCLAADVPL IESGTAGYLG QVTTIKKGVT 60
ECYECHPKPT QRTFPGCTIR NTPSEPIHCI VWAKYLFNQL FGEEDADQEV SPDRADPEAA 120
WEPTEAEARA RASNEDGDIK RISTKEWAKS TGYDPVKLFT KLFKDDIRYL LTMDKLWRKR 180
KPPVPLDWAE VQSQGEETNA SDQQNEPQLG LKDQQVLDVK SYARLFSKSI ETLRVHLAEK 240
GDGAELIWDK DDPSAMDFVT SAANLRMHIF SMNMKSRFDI KSMAGNIIPA IATTNAVIAG 300
LIVLEGLKIL SGKIDQCRTI FLNKQPNPRK KLLVPCALDP PNPNCYVCAS KPEVTVRLNV 360
HKVTVLTLQD KIVKEKFAMV APDVQIEDGK GTILISSEEG ETEANNHKKL SEFGIRNGSR 420
LQADDFLQDY TLLINILHSE DLGKDVEFEV VGDAPEKVGP KQAEDAAKSI TNGSDDGAQP 480
STSTAQEQDD VLIVDSDEED SSNNADVSEE ERSRKRKLDE KENLSAKRSR IEQKEELDDV 540
IALD 544 
Gene Ontology
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
 GO:0008641; F:small protein activating enzyme activity; IEA:InterPro.
 GO:0006464; P:cellular protein modification process; IEA:InterPro. 
Interpro
 IPR009036; Molybdenum_cofac_synth_MoeB.
 IPR016040; NAD(P)-bd_dom.
 IPR000594; ThiF_NAD_FAD-bd.
 IPR023280; Ub-like_act_enz_cat_cys_dom.
 IPR000127; UBact_repeat.
 IPR019572; Ubiquitin-activating_enzyme.
 IPR018074; UBQ-activ_enz_E1_AS. 
Pfam
 PF00899; ThiF
 PF10585; UBA_e1_thiolCys
 PF02134; UBACT 
SMART
  
PROSITE
 PS00536; UBIQUITIN_ACTIVAT_1
 PS00865; UBIQUITIN_ACTIVAT_2 
PRINTS