UniProt Accession

 SPP2A_HUMAN; Q8TCT8; B2RDS0; Q8TAW1; Q96SZ8 

Genbank Protein ID

 AJ345028; AY169314; AK027446; AK315651; AK075454; CH471082; BC025740; AJ420896 

Genbank Nucleotide ID

 CAC87789.1; AAO12539.1; BAB55117.1; BAG38017.1; BAC11630.1; EAW77410.1; AAH25740.1; CAD13133.1 

Protein Name

 Signal peptide peptidase-like 2A 

Protein Synonyms/Alias

 SPP-like 2A; SPPL2a; Intramembrane protease 3; IMP-3; Presenilin-like protein 2 

Gene Name

 SPPL2A 

Gene Synonyms/Alias

 IMP3; PSL2; PSEC0147 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
100	GSCHFLEKARIAQKG	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Intramembrane-cleaving aspartic protease (I-CLiP) that cleaves type II membrane signal peptides in the hydrophobic plane of the membrane. Functions in FASLG, ITM2B and TNF processing. Catalyzes the intramembrane cleavage of the anchored fragment of shed TNF-alpha (TNF), which promotes the release of the intracellular domain (ICD) for signaling to the nucleus. Also responsible for the intramembrane cleavage of Fas antigen ligand FASLG, which promotes the release of the intracellular FasL domain (FasL ICD). May play a role in the regulation of innate and adaptive immunity. 

Sequence Annotation

 DOMAIN 63 151 PA.
 MOTIF 463 465 PAL.
 MOTIF 495 498 YXXo lysosomal targeting motif.
 ACT_SITE 351 351 By similarity.
 ACT_SITE 412 412 By similarity.
 CARBOHYD 58 58 N-linked (GlcNAc...) (Potential).
 CARBOHYD 66 66 N-linked (GlcNAc...) (Potential).
 CARBOHYD 74 74 N-linked (GlcNAc...) (Potential).
 CARBOHYD 116 116 N-linked (GlcNAc...) (Potential).
 CARBOHYD 126 126 N-linked (GlcNAc...) (Potential).
 CARBOHYD 149 149 N-linked (GlcNAc...) (Potential).
 CARBOHYD 155 155 N-linked (GlcNAc...) (Potential).  

Keyword

 Cell membrane; Complete proteome; Endosome; Glycoprotein; Hydrolase; Lysosome; Membrane; Polymorphism; Protease; Reference proteome; Signal; Transmembrane; Transmembrane helix. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 520 AA 

Protein Sequence

Gene Ontology

 GO:0030660; C:Golgi-associated vesicle membrane; IDA:UniProtKB.
 GO:0071458; C:integral to cytosolic side of endoplasmic reticulum membrane; IDA:UniProtKB.
 GO:0071556; C:integral to lumenal side of endoplasmic reticulum membrane; IDA:UniProtKB.
 GO:0031902; C:late endosome membrane; IEA:UniProtKB-SubCell.
 GO:0005765; C:lysosomal membrane; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0004190; F:aspartic-type endopeptidase activity; IEA:InterPro.
 GO:0042803; F:protein homodimerization activity; IDA:UniProtKB.
 GO:0006509; P:membrane protein ectodomain proteolysis; IDA:UniProtKB.
 GO:0031293; P:membrane protein intracellular domain proteolysis; IDA:UniProtKB.
 GO:0050776; P:regulation of immune response; IMP:UniProtKB. 

Interpro

 IPR006639; Peptidase_A22.
 IPR007369; Peptidase_A22B_SPP.
 IPR003137; Protease-assoc_domain. 

Pfam

 PF02225; PA
 PF04258; Peptidase_A22B 

SMART

 SM00730; PSN 

PROSITE

  

PRINTS