CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-009967
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Receptor tyrosine-protein kinase erbB-2 
Protein Synonyms/Alias
 Proto-oncogene Neu; Proto-oncogene c-ErbB-2; p185erbB2; CD340 
Gene Name
 Erbb2 
Gene Synonyms/Alias
 Kiaa3023; Neu 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
725ETELRKLKVLGSGAFubiquitination[1]
748IPDGENVKIPVAIKVubiquitination[1]
938EIPDLLEKGERLPQPubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization (By similarity). 
Sequence Annotation
 DOMAIN 721 988 Protein kinase.
 NP_BIND 727 735 ATP (By similarity).
 REGION 677 690 Nuclear localization signal (By
 REGION 677 690 Required for interaction with KPNB1 and
 REGION 1196 1198 Interaction with PIK3C2B (By similarity).
 ACT_SITE 846 846 Proton acceptor (By similarity).
 BINDING 754 754 ATP (By similarity).
 MOD_RES 1055 1055 Phosphoserine (By similarity).
 MOD_RES 1108 1108 Phosphoserine (By similarity).
 MOD_RES 1140 1140 Phosphotyrosine; by autocatalysis (By
 MOD_RES 1197 1197 Phosphotyrosine (Potential).
 MOD_RES 1249 1249 Phosphotyrosine; by autocatalysis (By
 CARBOHYD 68 68 N-linked (GlcNAc...) (Potential).
 CARBOHYD 125 125 N-linked (GlcNAc...) (Potential).
 CARBOHYD 188 188 N-linked (GlcNAc...) (Potential).
 CARBOHYD 260 260 N-linked (GlcNAc...) (Potential).
 CARBOHYD 531 531 N-linked (GlcNAc...) (Potential).
 CARBOHYD 572 572 N-linked (GlcNAc...) (Potential).
 CARBOHYD 630 630 N-linked (GlcNAc...) (Potential).
 DISULFID 26 53 By similarity.
 DISULFID 163 193 By similarity.
 DISULFID 196 205 By similarity.
 DISULFID 200 213 By similarity.
 DISULFID 221 228 By similarity.
 DISULFID 225 236 By similarity.
 DISULFID 237 245 By similarity.
 DISULFID 241 253 By similarity.
 DISULFID 256 265 By similarity.
 DISULFID 269 296 By similarity.
 DISULFID 300 312 By similarity.
 DISULFID 316 332 By similarity.
 DISULFID 335 339 By similarity.
 DISULFID 343 368 By similarity.
 DISULFID 476 505 By similarity.
 DISULFID 512 521 By similarity.
 DISULFID 516 529 By similarity.
 DISULFID 532 541 By similarity.
 DISULFID 545 561 By similarity.
 DISULFID 564 577 By similarity.
 DISULFID 568 585 By similarity.
 DISULFID 588 597 By similarity.
 DISULFID 601 624 By similarity.
 DISULFID 627 635 By similarity.
 DISULFID 631 643 By similarity.  
Keyword
 Activator; ATP-binding; Cell membrane; Complete proteome; Cytoplasm; Disulfide bond; Glycoprotein; Kinase; Membrane; Nucleotide-binding; Nucleus; Phosphoprotein; Receptor; Reference proteome; Signal; Transcription; Transcription regulation; Transferase; Transmembrane; Transmembrane helix; Tyrosine-protein kinase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1256 AA 
Protein Sequence
MELAAWCRWG FLLALLSPGA AGTQVCTGTD MKLRLPASPE THLDMLRHLY QGCQVVQGNL 60
ELTYLPANAS LSFLQDIQEV QGYMLIAHNR VKHVPLQRLR IVRGTQLFED KYALAVLDNR 120
DPLDNVTTAA PGRTPEGLRE LQLRSLTEIL KGGVLIRGNP QLCYQDMVLW KDVLRKNNQL 180
APVDMDTNRS RACPPCAPTC KDNHCWGESP EDCQILTGTI CTSGCARCKG RLPTDCCHEQ 240
CAAGCTGPKH SDCLACLHFN HSGICELHCP ALITYNTDTF ESMLNPEGRY TFGASCVTTC 300
PYNYLSTEVG SCTLVCPPNN QEVTAEDGTQ RCEKCSKPCA GVCYGLGMEH LRGARAITSD 360
NIQEFAGCKK IFGSLAFLPE SFDGNPSSGV APLKPEHLQV FETLEEITGY LYISAWPESF 420
QDLSVFQNLR VIRGRILHDG AYSLTLQGLG IHSLGLRSLR ELGSGLALIH RNTHLCFVNT 480
VPWDQLFRNP HQALLHSGNR PEEACGLEGL VCNSLCARGH CWGPGPTQCV NCSQFLRGQE 540
CVEECRVWKG LPREYVRGKH CLPCHPECQP QNSSETCYGS EADQCEACAH YKDSSSCVAR 600
CPSGVKPDLS YMPIWKYPDE EGICQPCPIN CTHSCVDLDE RGCPAEQRAS PVTFIIATVV 660
GVLLFLIIVV VIGILIKRRR QKIRKYTMRR LLQETELVEP LTPSGAVPNQ AQMRILKETE 720
LRKLKVLGSG AFGTVYKGIW IPDGENVKIP VAIKVLRENT SPKANKEILD EAYVMAGVGS 780
PYVSRLLGIC LTSTVQLVTQ LMPYGCLLDH VREHRGRLGS QDLLNWCVQI AKGMSYLEEV 840
RLVHRDLAAR NVLVKSPNHV KITDFGLARL LDIDETEYHA DGGKVPIKWM ALESILRRRF 900
THQSDVWSYG VTVWELMTFG AKPYDGIPAR EIPDLLEKGE RLPQPPICTI DVYMIMVKCW 960
MIDSECRPRF RELVSEFSRM ARDPQRFVVI QNEDLGPSSP MDSTFYRSLL EDDDMGELVD 1020
AEEYLVPQQG FFSPDPALGT GSTAHRRHRS SSARSGGGEL TLGLEPSEEE PPRSPLAPSE 1080
GAGSDVFDGD LAVGVTKGLQ SLSPHDLSPL QRYSEDPTLP LPPETDGYVA PLACSPQPEY 1140
VNQPEVRPQS PLTPEGPPPP IRPAGATLER PKTLSPGKNG VVKDVFAFGG AVENPEYLAP 1200
RAGTASQPHP SPAFSPAFDN LYYWDQNSSE QGPPPSTFEG TPTAENPEYL GLDVPV 1256 
Gene Ontology
 GO:0016324; C:apical plasma membrane; IDA:MGI.
 GO:0016323; C:basolateral plasma membrane; IEA:Compara.
 GO:0005737; C:cytoplasm; IDA:MGI.
 GO:0010008; C:endosome membrane; IEA:Compara.
 GO:0016021; C:integral to membrane; NAS:UniProtKB.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
 GO:0043235; C:receptor complex; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004716; F:receptor signaling protein tyrosine kinase activity; IEA:InterPro.
 GO:0001042; F:RNA polymerase I core binding; ISS:UniProtKB.
 GO:0004714; F:transmembrane receptor protein tyrosine kinase activity; ISS:BHF-UCL.
 GO:0007507; P:heart development; IMP:MGI.
 GO:0008045; P:motor neuron axon guidance; IMP:MGI.
 GO:0042552; P:myelination; IMP:MGI.
 GO:0033088; P:negative regulation of immature T cell proliferation in thymus; IMP:MGI.
 GO:0007528; P:neuromuscular junction development; IMP:MGI.
 GO:0007422; P:peripheral nervous system development; IMP:MGI.
 GO:0014065; P:phosphatidylinositol 3-kinase cascade; IEA:Compara.
 GO:0045785; P:positive regulation of cell adhesion; IEA:Compara.
 GO:0030307; P:positive regulation of cell growth; ISS:UniProtKB.
 GO:0050679; P:positive regulation of epithelial cell proliferation; IEA:Compara.
 GO:0043406; P:positive regulation of MAP kinase activity; IEA:Compara.
 GO:0001934; P:positive regulation of protein phosphorylation; IMP:BHF-UCL.
 GO:0032321; P:positive regulation of Rho GTPase activity; IMP:BHF-UCL.
 GO:0045943; P:positive regulation of transcription from RNA polymerase I promoter; ISS:UniProtKB.
 GO:0045945; P:positive regulation of transcription from RNA polymerase III promoter; ISS:UniProtKB.
 GO:0045727; P:positive regulation of translation; ISS:UniProtKB.
 GO:0046777; P:protein autophosphorylation; IEA:Compara.
 GO:0070372; P:regulation of ERK1 and ERK2 cascade; ISS:UniProtKB.
 GO:0032886; P:regulation of microtubule-based process; ISS:UniProtKB.
 GO:0023014; P:signal transduction by phosphorylation; IEA:GOC.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0007169; P:transmembrane receptor protein tyrosine kinase signaling pathway; ISS:BHF-UCL.
 GO:0042060; P:wound healing; IEA:Compara. 
Interpro
 IPR000494; EGF_rcpt_L.
 IPR006211; Furin-like_Cys-rich_dom.
 IPR006212; Furin_repeat.
 IPR009030; Growth_fac_rcpt_N_dom.
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR001245; Ser-Thr/Tyr_kinase_cat_dom.
 IPR008266; Tyr_kinase_AS.
 IPR020635; Tyr_kinase_cat_dom.
 IPR016245; Tyr_kinase_EGF/ERB/XmrK_rcpt. 
Pfam
 PF00757; Furin-like
 PF07714; Pkinase_Tyr
 PF01030; Recep_L_domain 
SMART
 SM00261; FU
 SM00219; TyrKc 
PROSITE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00109; PROTEIN_KINASE_TYR 
PRINTS
 PR00109; TYRKINASE.