CPLM-001412

Genbank Nucleotide ID

Isocitrate dehydrogenase [NADP] cytoplasmic

Protein Synonyms/Alias

IDH; Cytosolic NADP-isocitrate dehydrogenase; IDP; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase

Mus musculus (Mouse)

Position	Peptide	Type	References
151	FVVPGPGKVEITYTP	acetylation	[1]
151	FVVPGPGKVEITYTP	ubiquitination	[2]
159	VEITYTPKDGTQKVT	acetylation	[3]
159	VEITYTPKDGTQKVT	ubiquitination	[2]
212	WPLYLSTKNTILKKY	ubiquitination	[2]
224	KKYDGRFKDIFQEIY	acetylation	[1, 3, 4, 5, 6, 7, 8, 9, 10]
224	KKYDGRFKDIFQEIY	phosphoglycerylation	[11]
224	KKYDGRFKDIFQEIY	ubiquitination	[2]
233	IFQEIYDKKYKSQFE	acetylation	[1, 3, 8, 10]
233	IFQEIYDKKYKSQFE	ubiquitination	[2]
236	EIYDKKYKSQFEAQK	ubiquitination	[2]
243	KSQFEAQKICYEHRL	acetylation	[3, 5, 6, 8, 10]
243	KSQFEAQKICYEHRL	ubiquitination	[2]
321	RHYRMYQKGQETSTN	ubiquitination	[2]
345	RGLAHRAKLDNNTEL	acetylation	[6]
345	RGLAHRAKLDNNTEL	ubiquitination	[2]
381	KDLAACIKGLPNVQR	ubiquitination	[2]
400	NTFEFMDKLGENLKA	acetylation	[1, 9]
400	NTFEFMDKLGENLKA	succinylation	[9]
400	NTFEFMDKLGENLKA	ubiquitination	[2]

[1] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
Simon GM, Cheng J, Gordon JI.
Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
[2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
[3] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
[4] Mitochondrial acetylome analysis in a mouse model of alcohol-induced liver injury utilizing SIRT3 knockout mice.
Fritz KS, Galligan JJ, Hirschey MD, Verdin E, Petersen DR.
J Proteome Res. 2012 Mar 2;11(3):1633-43. [PMID: 22309199]
[5] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
[6] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
PLoS One. 2012;7(12):e50545. [PMID: 23236377]
[7] Circadian acetylome reveals regulation of mitochondrial metabolic pathways.
Masri S, Patel VR, Eckel-Mahan KL, Peleg S, Forne I, Ladurner AG, Baldi P, Imhof A, Sassone-Corsi P.
Proc Natl Acad Sci U S A. 2013 Feb 26;110(9):3339-44. [PMID: 23341599]
[8] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
[9] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
[10] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
[11] Functional lysine modification by an intrinsically reactive primary glycolytic metabolite.
Moellering RE, Cravatt BF.
Science. 2013 Aug 2;341(6145):549-53. [PMID: 23908237]

Functional Description

NP_BIND 75 77 NADP (By similarity).
NP_BIND 310 315 NADP (By similarity).
REGION 94 100 Substrate binding (By similarity).
METAL 252 252 Magnesium or manganese (By similarity).
METAL 275 275 Magnesium or manganese (By similarity).
BINDING 77 77 Substrate (By similarity).
BINDING 82 82 NADP (By similarity).
BINDING 109 109 Substrate (By similarity).
BINDING 132 132 Substrate (By similarity).
BINDING 260 260 NADP (By similarity).
BINDING 328 328 NADP; via amide nitrogen and carbonyl
MOD_RES 321 321 N6-acetyllysine (By similarity).

3D-structure; Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Glyoxylate bypass; Magnesium; Manganese; Metal-binding; NADP; Oxidoreductase; Reference proteome; Tricarboxylic acid cycle.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005829; C:cytosol; IDA:MGI.
GO:0005739; C:mitochondrion; IDA:MGI.
GO:0005777; C:peroxisome; IEA:Compara.
GO:0004450; F:isocitrate dehydrogenase (NADP+) activity; ISS:UniProtKB.
GO:0000287; F:magnesium ion binding; ISS:UniProtKB.
GO:0051287; F:NAD binding; IEA:InterPro.
GO:0050661; F:NADP binding; IEA:Compara.
GO:0006103; P:2-oxoglutarate metabolic process; ISS:UniProtKB.
GO:0008585; P:female gonad development; IEA:Compara.
GO:0006749; P:glutathione metabolic process; IMP:MGI.
GO:0006097; P:glyoxylate cycle; IEA:UniProtKB-KW.
GO:0006102; P:isocitrate metabolic process; ISS:UniProtKB.
GO:0006979; P:response to oxidative stress; IDA:MGI.
GO:0048545; P:response to steroid hormone stimulus; IEA:Compara.
GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.

IPR019818; IsoCit/isopropylmalate_DH_CS.
IPR004790; Isocitrate_DH_NADP.
IPR024084; IsoPropMal-DH-like_dom.