CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006405
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Mannose-6-phosphate isomerase 
Protein Synonyms/Alias
 Phosphohexomutase; Phosphomannose isomerase; PMI 
Gene Name
 MPI 
Gene Synonyms/Alias
 PMI1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
44LAQIAEDKPYAELWMubiquitination[1]
60THPRGDAKILDNRISubiquitination[1]
69LDNRISQKTLSQWIAubiquitination[1, 2, 3]
85NQDSLGSKVKDTFNGubiquitination[1, 4]
87DSLGSKVKDTFNGNLubiquitination[1]
115SIQAHPNKELAEKLHubiquitination[1]
163EIVTFLKKVPEFQFLubiquitination[1]
218EQLNLLVKRISQQAAubiquitination[1, 4]
320SYTPSSSKDRLFLPTubiquitination[1]
349VPDFTIMKTEVPGSVubiquitination[4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions. 
Sequence Annotation
 ACT_SITE 295 295 By similarity.
 METAL 110 110 Zinc (By similarity).
 METAL 112 112 Zinc (By similarity).
 METAL 137 137 Zinc (By similarity).
 METAL 276 276 Zinc (By similarity).
 MOD_RES 2 2 N-acetylalanine.  
Keyword
 Acetylation; Alternative splicing; Complete proteome; Congenital disorder of glycosylation; Cytoplasm; Direct protein sequencing; Disease mutation; Isomerase; Metal-binding; Reference proteome; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 423 AA 
Protein Sequence
MAAPRVFPLS CAVQQYAWGK MGSNSEVARL LASSDPLAQI AEDKPYAELW MGTHPRGDAK 60
ILDNRISQKT LSQWIAENQD SLGSKVKDTF NGNLPFLFKV LSVETPLSIQ AHPNKELAEK 120
LHLQAPQHYP DANHKPEMAI ALTPFQGLCG FRPVEEIVTF LKKVPEFQFL IGDEAATHLK 180
QTMSHDSQAV ASSLQSCFSH LMKSEKKVVV EQLNLLVKRI SQQAAAGNNM EDIFGELLLQ 240
LHQQYPGDIG CFAIYFLNLL TLKPGEAMFL EANVPHAYLK GDCVECMACS DNTVRAGLTP 300
KFIDVPTLCE MLSYTPSSSK DRLFLPTRSQ EDPYLSIYDP PVPDFTIMKT EVPGSVTEYK 360
VLALDSASIL LMVQGTVIAS TPTTQTPIPL QRGGVLFIGA NESVSLKLTE PKDLLIFRAC 420
CLL 423 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0004476; F:mannose-6-phosphate isomerase activity; TAS:ProtInc.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0006488; P:dolichol-linked oligosaccharide biosynthetic process; TAS:Reactome.
 GO:0009298; P:GDP-mannose biosynthetic process; TAS:Reactome.
 GO:0043687; P:post-translational protein modification; TAS:Reactome.
 GO:0018279; P:protein N-linked glycosylation via asparagine; TAS:Reactome. 
Interpro
 IPR001250; Man6P_Isoase-1.
 IPR016305; Mannose-6-P_Isomerase.
 IPR018050; Pmannose_isomerase-type1_CS.
 IPR014710; RmlC-like_jellyroll.
 IPR011051; RmlC_Cupin. 
Pfam
 PF01238; PMI_typeI 
SMART
  
PROSITE
 PS00965; PMI_I_1
 PS00966; PMI_I_2 
PRINTS
 PR00714; MAN6PISMRASE.