UniProt Accession

 DUS4_HUMAN; Q13115; B2RBU5; D3DSU4; G5E930; Q13524 

Genbank Protein ID

 U21108; U48807; AK314820; AL137704; AC084262; CH471080; CH471080; CH471080; CH471080; BC002671; BC014565 

Genbank Nucleotide ID

 AAA85119.1; AAC50452.1; BAG37342.1; EAW63481.1; EAW63482.1; EAW63483.1; EAW63484.1; AAH02671.1; AAH14565.1 

Protein Name

 Dual specificity protein phosphatase 4 

Protein Synonyms/Alias

 Dual specificity protein phosphatase hVH2; Mitogen-activated protein kinase phosphatase 2; MAP kinase phosphatase 2; MKP-2 

Gene Name

 DUSP4 

Gene Synonyms/Alias

 MKP2; VH2 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
78	TIVRRRAKGSVSLEQ	ubiquitination	[1, 2]
144	RTDICLLKGGYERFS	ubiquitination	[3]
160	EYPEFCSKTKALAAI	ubiquitination	[3]
311	EEAFEFVKQRRSIIS	ubiquitination	[1, 2, 3]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 

Functional Description

 Regulates mitogenic signal transduction by dephosphorylating both Thr and Tyr residues on MAP kinases ERK1 and ERK2. 

Sequence Annotation

 DOMAIN 41 159 Rhodanese.
 DOMAIN 197 394 Tyrosine-protein phosphatase.
 ACT_SITE 280 280 Phosphocysteine intermediate (By
 MOD_RES 386 386 Phosphoserine; by MAPK.
 MOD_RES 391 391 Phosphoserine; by MAPK.  

Keyword

 3D-structure; Alternative splicing; Complete proteome; Hydrolase; Nucleus; Phosphoprotein; Protein phosphatase; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 394 AA 

Protein Sequence

Gene Ontology

 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0017017; F:MAP kinase tyrosine/serine/threonine phosphatase activity; IBA:RefGenome.
 GO:0004725; F:protein tyrosine phosphatase activity; IEA:EC.
 GO:0008330; F:protein tyrosine/threonine phosphatase activity; TAS:ProtInc.
 GO:0001706; P:endoderm formation; IBA:RefGenome.
 GO:0045087; P:innate immune response; TAS:Reactome.
 GO:0002755; P:MyD88-dependent toll-like receptor signaling pathway; TAS:Reactome.
 GO:0048011; P:neurotrophin TRK receptor signaling pathway; TAS:Reactome.
 GO:0035335; P:peptidyl-tyrosine dephosphorylation; IEA:GOC.
 GO:0051403; P:stress-activated MAPK cascade; TAS:Reactome.
 GO:0034166; P:toll-like receptor 10 signaling pathway; TAS:Reactome.
 GO:0034134; P:toll-like receptor 2 signaling pathway; TAS:Reactome.
 GO:0034138; P:toll-like receptor 3 signaling pathway; TAS:Reactome.
 GO:0034142; P:toll-like receptor 4 signaling pathway; TAS:Reactome.
 GO:0034146; P:toll-like receptor 5 signaling pathway; TAS:Reactome.
 GO:0034162; P:toll-like receptor 9 signaling pathway; TAS:Reactome.
 GO:0038123; P:toll-like receptor TLR1:TLR2 signaling pathway; TAS:Reactome.
 GO:0038124; P:toll-like receptor TLR6:TLR2 signaling pathway; TAS:Reactome.
 GO:0035666; P:TRIF-dependent toll-like receptor signaling pathway; TAS:Reactome. 

Interpro

 IPR000340; Dual-sp_phosphatase_cat-dom.
 IPR020422; Dual-sp_phosphatase_subgr_cat.
 IPR024950; DUSP.
 IPR008343; MKP.
 IPR001763; Rhodanese-like_dom.
 IPR000387; Tyr/Dual-sp_Pase.
 IPR016130; Tyr_Pase_AS. 

Pfam

 PF00782; DSPc
 PF00581; Rhodanese 

SMART

 SM00195; DSPc
 SM00450; RHOD 

PROSITE

 PS50206; RHODANESE_3
 PS00383; TYR_PHOSPHATASE_1
 PS50056; TYR_PHOSPHATASE_2
 PS50054; TYR_PHOSPHATASE_DUAL 

PRINTS

 PR01764; MAPKPHPHTASE.