CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019319
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 H/ACA ribonucleoprotein complex non-core subunit NAF1 
Protein Synonyms/Alias
 hNAF1 
Gene Name
 NAF1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
265SSDHIESKGIKIKETubiquitination[1]
270ESKGIKIKETMYFAPubiquitination[1]
290TQYIFTEKLKQDKGSubiquitination[1, 2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 RNA-binding protein required for the maturation of box H/ACA snoRNPs complex and ribosome biogenesis. During assembly of the H/ACA snoRNPs complex, it associates with the complex and disappears during maturation of the complex and is replaced by NOLA1/GAR1 to yield mature H/ACA snoRNPs complex. Probably competes with NOLA1/GAR1 for binding with DKC1/NOLA4. 
Sequence Annotation
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 315 315 Phosphoserine.  
Keyword
 3D-structure; Acetylation; Alternative splicing; Complete proteome; Cytoplasm; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Ribonucleoprotein; Ribosome biogenesis; RNA-binding; rRNA processing. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 494 AA 
Protein Sequence
MEVVEAAAAQ LETLKFNGTD FGVGEGPAAP SPGSAPVPGT QPPLQSFEGS PDAGQTVEVK 60
PAGEQPLQPV LNAVAAGTPA PQPQPPAESP ACGDCVTSPG AAEPARAPDS LETSDSDSDS 120
DSETDSDSSS SSSSSSSSSS SSSSSCISLP PVLSDGDDDL QIEKENKNFP LKTKDELLLN 180
ELPSVEELTI ILPEDIELKP LGMVSSIIEQ LVIIESMTNL PPVNEETVIF KSDRQAAGKI 240
FEIFGPVAHP FYVLRFNSSD HIESKGIKIK ETMYFAPSMK DFTQYIFTEK LKQDKGSDAS 300
WKNDQEPPPE ALDFSDDEKE KEAKQRKKSQ IQGRKKLKSE FNEPGEDFTE VHQNWNAHSS 360
ASEHAKGYRN REFTRGFSRA RYPRSCHGRP PPQHFYNSEH MVSQETSGFP SQRQNNPIMP 420
QYPFPLPVFD MHNFPLRPPP PPPPPPVNMG WATPNMAAHP LLNLPYSLPP PPPPPPLPPP 480
PSSGDSNSHF GPYY 494 
Gene Ontology
 GO:0030054; C:cell junction; IDA:HPA.
 GO:0005813; C:centrosome; IDA:HPA.
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0005732; C:small nucleolar ribonucleoprotein complex; IDA:UniProtKB.
 GO:0003723; F:RNA binding; IDA:UniProtKB.
 GO:0030515; F:snoRNA binding; IEA:InterPro.
 GO:0042254; P:ribosome biogenesis; IDA:UniProtKB.
 GO:0006364; P:rRNA processing; IEA:UniProtKB-KW.
 GO:0031120; P:snRNA pseudouridine synthesis; IEA:InterPro. 
Interpro
 IPR007504; H/ACA_rnp_Gar1/Naf1.
 IPR009000; Transl_elong_init/rib_B-barrel. 
Pfam
 PF04410; Gar1 
SMART
  
PROSITE
  
PRINTS