UniProt Accession

 NUOI_ECOLI; P0AFD6; P33604; P76488; P78183 

Genbank Protein ID

 X68301; U00096; AP009048 

Genbank Nucleotide ID

 CAA48368.1; AAC75341.1; BAA16109.2 

Protein Name

 NADH-quinone oxidoreductase subunit I 

Protein Synonyms/Alias

 NADH dehydrogenase I subunit I; NDH-1 subunit I; NUO9 

Gene Name

 nuoI 

Gene Synonyms/Alias

 b2281; JW2276 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
83	SLQKAETKDGRWYPE	acetylation	[1]
134	RQDLVYEKEDLLISG	acetylation	[1]
171	GEAENEAKPIDVKSL	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient. 

Sequence Annotation

 DOMAIN 50 80 4Fe-4S ferredoxin-type 1.
 DOMAIN 90 119 4Fe-4S ferredoxin-type 2.
 METAL 60 60 Iron-sulfur 1 (4Fe-4S) (By similarity).
 METAL 63 63 Iron-sulfur 1 (4Fe-4S) (By similarity).
 METAL 66 66 Iron-sulfur 1 (4Fe-4S) (By similarity).
 METAL 70 70 Iron-sulfur 2 (4Fe-4S) (By similarity).
 METAL 99 99 Iron-sulfur 2 (4Fe-4S) (By similarity).
 METAL 102 102 Iron-sulfur 2 (4Fe-4S) (By similarity).
 METAL 105 105 Iron-sulfur 2 (4Fe-4S) (By similarity).
 METAL 109 109 Iron-sulfur 1 (4Fe-4S) (By similarity).  

Keyword

 4Fe-4S; Cell inner membrane; Cell membrane; Complete proteome; Direct protein sequencing; Iron; Iron-sulfur; Membrane; Metal-binding; NAD; Oxidoreductase; Quinone; Reference proteome; Repeat; Ubiquinone. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 180 AA 

Protein Sequence

Gene Ontology

 GO:0045272; C:plasma membrane respiratory chain complex I; IDA:EcoCyc.
 GO:0051539; F:4 iron, 4 sulfur cluster binding; IDA:EcoCyc.
 GO:0009055; F:electron carrier activity; IEA:InterPro.
 GO:0005506; F:iron ion binding; IEA:HAMAP.
 GO:0050136; F:NADH dehydrogenase (quinone) activity; IEA:HAMAP.
 GO:0048038; F:quinone binding; IEA:UniProtKB-KW.
 GO:0009060; P:aerobic respiration; IMP:EcoCyc.
 GO:0019684; P:photosynthesis, light reaction; IEA:HAMAP. 

Interpro

 IPR001450; 4Fe4S-bd_dom.
 IPR017896; 4Fe4S_Fe-S-bd.
 IPR017900; 4Fe4S_Fe_S_CS.
 IPR010226; NADH_quinone_OxRdtase_chainI. 

Pfam

 PF00037; Fer4 

SMART

  

PROSITE

 PS00198; 4FE4S_FER_1
 PS51379; 4FE4S_FER_2 

PRINTS