UniProt Accession

 SF3A3_MOUSE; Q9D554 

Genbank Protein ID

 BC009141; AK015776 

Genbank Nucleotide ID

 AAH09141.1; BAB29971.1 

Protein Name

 Splicing factor 3A subunit 3 

Protein Synonyms/Alias

 SF3a60; Spliceosome-associated protein 61; SAP 61 

Gene Name

 Sf3a3 

Gene Synonyms/Alias

 Sap61 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
97	QIKEFHRKHPNEICV	acetylation	[1]
201	QDYTDRVKPLQDQNE	ubiquitination	[2]
212	DQNELFGKIQTDFEK	acetylation	[1]
220	IQTDFEKKWDNGTFP	ubiquitination	[2]
264	SLGLDRLKSALLALG	acetylation	[1]
291	RLFSTKGKSLESLDT	acetylation	[1]
303	LDTSLFAKNPKSKGT	acetylation	[1, 3]
303	LDTSLFAKNPKSKGT	ubiquitination	[2]

Reference

 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 

Functional Description

 Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex (By similarity). 

Sequence Annotation

 ZN_FING 406 437 Matrin-type.
 MOD_RES 1 1 N-acetylmethionine (By similarity).
 MOD_RES 299 299 Phosphoserine (By similarity).
 MOD_RES 365 365 Phosphoserine (By similarity).
 MOD_RES 367 367 Phosphoserine (By similarity).
 MOD_RES 369 369 Phosphoserine (By similarity).  

Keyword

 Acetylation; Complete proteome; Metal-binding; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein; Reference proteome; Spliceosome; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 501 AA 

Protein Sequence

Gene Ontology

 GO:0071013; C:catalytic step 2 spliceosome; IEA:Compara.
 GO:0003676; F:nucleic acid binding; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
 GO:0008380; P:RNA splicing; IEA:UniProtKB-KW. 

Interpro

 IPR024598; DUF3449.
 IPR021966; SF3a60_bindingd.
 IPR015880; Znf_C2H2-like.
 IPR000690; Znf_C2H2_matrin. 

Pfam

 PF11931; DUF3449
 PF12108; SF3a60_bindingd 

SMART

 SM00355; ZnF_C2H2 

PROSITE

 PS50171; ZF_MATRIN 

PRINTS