CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-013285
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 NAD kinase 2, mitochondrial 
Protein Synonyms/Alias
 Mitochondrial NAD kinase; NAD kinase domain-containing protein 1, mitochondrial 
Gene Name
 NADK2 
Gene Synonyms/Alias
 C5orf33; MNADK; NADKD1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
98ELSEEDLKQLLALKGubiquitination[1]
104LKQLLALKGSSYSGLubiquitination[2, 3]
170TMLLAASKVLDRLKPubiquitination[4]
302VDDGPWEKQKSSGLNubiquitination[5]
317LCTGTGSKAWSFNINubiquitination[4]
339EDVLNIAKRQGNLSLubiquitination[4, 5]
Reference
 [1] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Mitochondrial NAD(+) kinase that phosphorylates NAD(+) to yield NADP(+). Can use both ATP or inorganic polyphosphate as the phosphoryl donor. Also has weak NADH kinase activity in vitro; however NADH kinase activity is much weaker than the NAD(+) kinase activity and may not be relevant in vivo. 
Sequence Annotation
 MOD_RES 188 188 Phosphoserine (By similarity).  
Keyword
 Alternative splicing; ATP-binding; Complete proteome; Kinase; Mitochondrion; NAD; NADP; Nucleotide-binding; Phosphoprotein; Reference proteome; Transferase; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 442 AA 
Protein Sequence
MTCYRGFLLG SCCRVAGGRA AALRGPGAGG PAARPRLGGD GGGRRHLGQG QPRELAGCGS 60
RADGGFRPSR VVVVAKTTRY EFEQQRYRYA ELSEEDLKQL LALKGSSYSG LLERHHIHTK 120
NVEHIIDSLR NEGIEVRLVK RREYDEETVR WADAVIAAGG DGTMLLAASK VLDRLKPVIG 180
VNTDPERSEG HLCLPVRYTH SFPEALQKFY RGEFRWLWRQ RIRLYLEGTG INPVPVDLHE 240
QQLSLNQHNR ALNIERAHDE RSEASGPQLL PVRALNEVFI GESLSSRASY YEISVDDGPW 300
EKQKSSGLNL CTGTGSKAWS FNINRVATQA VEDVLNIAKR QGNLSLPLNR ELVEKVTNEY 360
NESLLYSPEE PKILFSIREP IANRVFSSSR QRCFSSKVCV RSRCWDACMV VDGGTSFEFN 420
DGAIASMMIN KEDELRTVLL EQ 442 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0003951; F:NAD+ kinase activity; IDA:UniProtKB.
 GO:0042803; F:protein homodimerization activity; IDA:UniProtKB.
 GO:0019674; P:NAD metabolic process; IDA:UniProtKB.
 GO:0006741; P:NADP biosynthetic process; IEA:InterPro. 
Interpro
 IPR012355; ATP-NAD-like_euk.
 IPR017438; ATP-NAD_kinase_dom_1.
 IPR016064; ATP-NAD_kinase_PpnK-typ.
 IPR017437; ATP-NAD_kinase_PpnK-typ_all-b.
 IPR002504; PolyP/ATP_NADK_prd. 
Pfam
 PF01513; NAD_kinase 
SMART
  
PROSITE
  
PRINTS