UniProt Accession

 VPS74_YEAST; Q06385; D6VT03 

Genbank Protein ID

 U28373; BK006938 

Genbank Nucleotide ID

 AAB64808.1; DAA12213.1 

Protein Name

 Vacuolar protein sorting-associated protein 74 

Protein Synonyms/Alias

  

Gene Name

 VPS74 

Gene Synonyms/Alias

 YDR372C; D9481.15 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
28	HSSANNTKGDKIANI	ubiquitination	[1]
31	ANNTKGDKIANIAVD	ubiquitination	[1]
47	DDDNGTNKKIAYDPE	ubiquitination	[2]
48	DDNGTNKKIAYDPEE	ubiquitination	[1]
57	AYDPEESKLRDNINI	ubiquitination	[1, 2]
217	PIADASCKEAIKRRV	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 

Functional Description

 Mediates the cis and medial Golgi localization of mannosyltransferases through direct binding of their cytosolic domains. Involved in vacuolar protein sorting. 

Sequence Annotation

 REGION 197 208 Beta-hairpin required for
 BINDING 88 88 PtdIns4P (Probable).
 BINDING 97 97 PtdIns4P (Probable).
 BINDING 178 178 PtdIns4P (Probable).
 BINDING 181 181 PtdIns4P (Probable).
 MOD_RES 14 14 Phosphoserine.  

Keyword

 3D-structure; Complete proteome; Golgi apparatus; Lipid-binding; Membrane; Phosphoprotein; Protein transport; Reference proteome; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 345 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:SGD.
 GO:0019898; C:extrinsic to membrane; IDA:SGD.
 GO:0005794; C:Golgi apparatus; IDA:SGD.
 GO:0032580; C:Golgi cisterna membrane; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0070273; F:phosphatidylinositol-4-phosphate binding; IMP:SGD.
 GO:0030968; P:endoplasmic reticulum unfolded protein response; IMP:SGD.
 GO:0034067; P:protein localization to Golgi apparatus; IMP:SGD.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW.
 GO:0006890; P:retrograde vesicle-mediated transport, Golgi to ER; IMP:SGD. 

Interpro

 IPR008628; GPP34. 

Pfam

 PF05719; GPP34 

SMART

  

PROSITE

  

PRINTS