CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018816
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Signal transducing adapter molecule 1 
Protein Synonyms/Alias
 STAM-1 
Gene Name
 STAM 
Gene Synonyms/Alias
 STAM1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
99EVSNVLNKGHPKVCEubiquitination[1, 2, 3]
103VLNKGHPKVCEKLKAubiquitination[2]
133SLISAMIKNLKEQGVubiquitination[4, 5, 6]
136SAMIKNLKEQGVTFPubiquitination[2]
154SQAAEQAKASPALVAubiquitination[2, 4, 5, 6]
162ASPALVAKDPGTVANubiquitination[5, 7]
170DPGTVANKKEEEDLAubiquitination[2, 5]
171PGTVANKKEEEDLAKubiquitination[5]
185KAIELSLKEQRQQSTubiquitination[2, 4, 5, 6, 8]
276TAEPEMIKTEKKTVQubiquitination[5]
Reference
 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [7] Global analysis of lysine ubiquitination by ubiquitin remnant immunoaffinity profiling.
 Xu G, Paige JS, Jaffrey SR.
 Nat Biotechnol. 2010 Aug;28(8):868-73. [PMID: 20639865]
 [8] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572] 
Functional Description
 Involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. May also play a role in T-cell development. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with HGS (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. 
Sequence Annotation
 DOMAIN 16 143 VHS.
 REPEAT 171 190 UIM.
 DOMAIN 210 269 SH3.
 DOMAIN 370 387 ITAM.
 MOD_RES 156 156 Phosphoserine.
 MOD_RES 198 198 Phosphotyrosine.
 MOD_RES 381 381 Phosphotyrosine.
 MOD_RES 384 384 Phosphotyrosine.  
Keyword
 3D-structure; Alternative splicing; Complete proteome; Cytoplasm; Direct protein sequencing; Endosome; Membrane; Phosphoprotein; Polymorphism; Protein transport; Reference proteome; SH3 domain; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 540 AA 
Protein Sequence
MPLFATNPFD QDVEKATSEM NTAEDWGLIL DICDKVGQSR TGPKDCLRSI MRRVNHKDPH 60
VAMQALTLLG ACVSNCGKIF HLEVCSRDFA SEVSNVLNKG HPKVCEKLKA LMVEWTDEFK 120
NDPQLSLISA MIKNLKEQGV TFPAIGSQAA EQAKASPALV AKDPGTVANK KEEEDLAKAI 180
ELSLKEQRQQ STTLSTLYPS TSSLLTNHQH EGRKVRAIYD FEAAEDNELT FKAGEIITVL 240
DDSDPNWWKG ETHQGIGLFP SNFVTADLTA EPEMIKTEKK TVQFSDDVQV ETIEPEPEPA 300
FIDEDKMDQL LQMLQSTDPS DDQPDLPELL HLEAMCHQMG PLIDEKLEDI DRKHSELSEL 360
NVKVMEALSL YTKLMNEDPM YSMYAKLQNQ PYYMQSSGVS GSQVYAGPPP SGAYLVAGNA 420
QMSHLQSYSL PPEQLSSLSQ AVVPPSANPA LPSQQTQAAY PNTMVSSVQG NTYPSQAPVY 480
SPPPAATAAA ATADVTLYQN AGPNMPQVPN YNLTSSTLPQ PGGSQQPPQP QQPYSQKALL 540 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0031901; C:early endosome membrane; IEA:UniProtKB-SubCell.
 GO:0005070; F:SH3/SH2 adaptor activity; TAS:ProtInc.
 GO:0016044; P:cellular membrane organization; TAS:Reactome.
 GO:0016197; P:endosomal transport; TAS:Reactome.
 GO:0007173; P:epidermal growth factor receptor signaling pathway; TAS:Reactome.
 GO:0006886; P:intracellular protein transport; IEA:InterPro.
 GO:0042059; P:negative regulation of epidermal growth factor receptor signaling pathway; TAS:Reactome. 
Interpro
 IPR008942; ENTH_VHS.
 IPR000108; p67phox.
 IPR001452; SH3_domain.
 IPR003903; Ubiquitin-int_motif.
 IPR002014; VHS.
 IPR018205; VHS_subgr. 
Pfam
 PF00018; SH3_1
 PF02809; UIM
 PF00790; VHS 
SMART
 SM00326; SH3
 SM00726; UIM
 SM00288; VHS 
PROSITE
 PS51055; ITAM_1
 PS50002; SH3
 PS50330; UIM
 PS50179; VHS 
PRINTS
 PR00499; P67PHOX.
 PR00452; SH3DOMAIN.