CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023062
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 ADP-ribosylation factor-binding protein GGA1 
Protein Synonyms/Alias
 Gamma-adaptin-related protein 1; Golgi-localized, gamma ear-containing, ARF-binding protein 1 
Gene Name
 GGA1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
54ATRLLAHKIQSPQEWubiquitination[1]
132PLPPPRPKNVIFEDEubiquitination[2]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 
Functional Description
 Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF- dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif. 
Sequence Annotation
 DOMAIN 17 147 VHS.
 DOMAIN 171 299 GAT.
 DOMAIN 510 631 GAE.
 REGION 114 274 Interaction with ARF3.
 REGION 300 509 Unstructured hinge.
 MOTIF 358 362 Autoinhibitory.
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 355 355 Phosphoserine; by CK2 (Probable).  
Keyword
 3D-structure; Acetylation; Alternative splicing; Complete proteome; Endosome; Golgi apparatus; Membrane; Phosphoprotein; Polymorphism; Protein transport; Reference proteome; Transport; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 639 AA 
Protein Sequence
MEPAMEPETL EARINRATNP LNKELDWASI NGFCEQLNED FEGPPLATRL LAHKIQSPQE 60
WEAIQALTYL GSRTSEKVKN KILELLYSWT VGLPEEVKIA EAYQMLKKQG IVKSDPKLPD 120
DTTFPLPPPR PKNVIFEDEE KSKMLARLLK SSHPEDLRAA NKLIKEMVQE DQKRMEKISK 180
RVNAIEEVNN NVKLLTEMVM SHSQGGAAAG SSEDLMKELY QRCERMRPTL FRLASDTEDN 240
DEALAEILQA NDNLTQVINL YKQLVRGEEV NGDATAGSIP GSTSALLDLS GLDLPPAGTT 300
YPAMPTRPGE QASPEQPSAS VSLLDDELMS LGLSDPTPPS GPSLDGTGWN SFQSSDATEP 360
PAPALAQAPS MESRPPAQTS LPASSGLDDL DLLGKTLLQQ SLPPESQQVR WEKQQPTPRL 420
TLRDLQNKSS SCSSPSSSAT SLLHTVSPEP PRPPQQPVPT ELSLASITVP LESIKPSNIL 480
PVTVYDQHGF RILFHFARDP LPGRSDVLVV VVSMLSTAPQ PIRNIVFQSA VPKVMKVKLQ 540
PPSGTELPAF NPIVHPSAIT QVLLLANPQK EKVRLRYKLT FTMGDQTYNE MGDVDQFPPP 600
ETWGSL 606 
Gene Ontology
 GO:0030131; C:clathrin adaptor complex; IEA:InterPro.
 GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
 GO:0005794; C:Golgi apparatus; TAS:UniProtKB.
 GO:0006886; P:intracellular protein transport; TAS:UniProtKB.
 GO:0016192; P:vesicle-mediated transport; IEA:InterPro. 
Interpro
 IPR008152; Clathrin_a/b/g-adaptin_app_Ig.
 IPR008153; Clathrin_g-adaptin_app.
 IPR013041; Coatomer/clathrin_app_Ig-like.
 IPR008942; ENTH_VHS.
 IPR004152; GAT.
 IPR002014; VHS.
 IPR018205; VHS_subgr. 
Pfam
 PF02883; Alpha_adaptinC2
 PF03127; GAT
 PF00790; VHS 
SMART
 SM00809; Alpha_adaptinC2
 SM00288; VHS 
PROSITE
 PS50180; GAE
 PS50909; GAT
 PS50179; VHS 
PRINTS