UniProt Accession

 SEN2_HUMAN; Q8NCE0; B7Z6K1; C9IZI7; G5E9Q3; Q8WTW7; Q9BPU7 

Genbank Protein ID

 AK074794; AK300449; AK225875; AC018500; AC090947; CH471055; BC004178; BC004211; BC019582; BC021975 

Genbank Nucleotide ID

 BAC11213.1; BAH13287.1; EAW64129.1; AAH04178.1; AAH04211.1; AAH19582.1; AAH21975.2 

Protein Name

 tRNA-splicing endonuclease subunit Sen2 

Protein Synonyms/Alias

 tRNA-intron endonuclease Sen2; HsSen2 

Gene Name

 TSEN2 

Gene Synonyms/Alias

 SEN2 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
63	DIEQLYGKGYFGKGI	ubiquitination	[1]
68	YGKGYFGKGILSRSR	ubiquitination	[1]
208	PTTESFEKSVREDAS	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. Isoform 1 probably carries the active site for 5'-splice site cleavage. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre- mRNA 3'-end processing factors, establishing a link between pre- tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events. Isoform 2 is responsible for processing a yet unknown RNA substrate. The complex containing isoform 2 is not able to cleave pre-tRNAs properly, although it retains endonucleolytic activity. 

Sequence Annotation

 ACT_SITE 369 369 By similarity.
 ACT_SITE 377 377 By similarity.
 ACT_SITE 416 416 By similarity.
 MOD_RES 408 408 Phosphoserine (By similarity).
 MOD_RES 411 411 Phosphoserine (By similarity).
 MOD_RES 415 415 Phosphoserine (By similarity).  

Keyword

 Alternative splicing; Complete proteome; Endonuclease; Hydrolase; mRNA processing; Nuclease; Nucleus; Phosphoprotein; Polymorphism; Pontocerebellar hypoplasia; Reference proteome; tRNA processing. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 465 AA 

Protein Sequence

Gene Ontology

 GO:0005813; C:centrosome; IDA:HPA.
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0000214; C:tRNA-intron endonuclease complex; IEA:InterPro.
 GO:0003676; F:nucleic acid binding; IEA:InterPro.
 GO:0000213; F:tRNA-intron endonuclease activity; IEA:EC.
 GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
 GO:0090305; P:nucleic acid phosphodiester bond hydrolysis; IEA:GOC.
 GO:0006388; P:tRNA splicing, via endonucleolytic cleavage and ligation; IDA:UniProtKB. 

Interpro

 IPR011856; tRNA_endonuc-like_dom.
 IPR006677; tRNA_intron_Endonuc_cat-like.
 IPR006678; tRNA_intron_Endonuc_N.
 IPR016589; tRNA_splic_SEN2. 

Pfam

 PF01974; tRNA_int_endo
 PF02778; tRNA_int_endo_N 

SMART

  

PROSITE

  

PRINTS