CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-042638
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Mediator of RNA polymerase II transcription subunit 28 
Protein Synonyms/Alias
  
Gene Name
 MED28 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
80GVDQCIQKFLDIARQubiquitination[1, 2, 3]
95TECFFLQKRLQLSVQubiquitination[2]
128RKDALVQKHLTKLRHubiquitination[2, 3]
173ANIPAPLKPTHLTG*acetylation[3, 4]
Reference
 [1] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 179 AA 
Protein Sequence
PLGGMFSGQP PGPPQAPPGL PGQASLLQAA PGAPRPSSST LVDELESSFE ACFASLVSQD 60
YVNGTDQEEI RTGVDQCIQK FLDIARQTEC FFLQKRLQLS VQKPEQVIKE DVSELRNELQ 120
RKDALVQKHL TKLRHWQQVL EDINVQHKKP ADIPQGSLAY LEQASANIPA PLKPTHLTG 179 
Gene Ontology
 GO:0005634; C:nucleus; IDA:HPA. 
Interpro
 IPR021640; Mediator_Med28. 
Pfam
 PF11594; Med28 
SMART
  
PROSITE
  
PRINTS