CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021591
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 PR domain zinc finger protein 16 
Protein Synonyms/Alias
 PR domain-containing protein 16; Transcription factor MEL1; MDS1/EVI1-like gene 1 
Gene Name
 PRDM16 
Gene Synonyms/Alias
 KIAA1675; MEL1; PFM13 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
752LAHNLLVKAEPKSPRsumoylation[1]
778CPFDLTTKPKDVKPIubiquitination[2]
Reference
 [1] Sumoylation of MEL1S at lysine 568 and its interaction with CtBP facilitates its repressor activity and the blockade of G-CSF-induced myeloid differentiation.
 Nishikata I, Nakahata S, Saito Y, Kaneda K, Ichihara E, Yamakawa N, Morishita K.
 Oncogene. 2011 Oct 6;30(40):4194-207. [PMID: 21516122]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Binds DNA and functions as a transcriptional regulator. Functions in the differentiation of brown adipose tissue (BAT) which is specialized in dissipating chemical energy in the form of heat in response to cold or excess feeding while white adipose tissue (WAT) is specialized in the storage of excess energy and the control of systemic metabolism. Together with CEBPB, regulates the differentiation of myoblastic precursors into brown adipose cells. Functions also as a repressor of TGF-beta signaling. Isoform 4 may regulate granulocytes differentiation. 
Sequence Annotation
 DOMAIN 82 211 SET.
 ZN_FING 230 253 C2H2-type 1; atypical.
 ZN_FING 281 303 C2H2-type 2.
 ZN_FING 309 331 C2H2-type 3.
 ZN_FING 337 360 C2H2-type 4.
 ZN_FING 366 388 C2H2-type 5.
 ZN_FING 394 416 C2H2-type 6.
 ZN_FING 423 445 C2H2-type 7; atypical.
 ZN_FING 951 973 C2H2-type 8.
 ZN_FING 979 1002 C2H2-type 9.
 ZN_FING 1008 1032 C2H2-type 10.
 REGION 679 1038 Interaction with CTBP1 and CTBP2 (By
 REGION 739 1276 Mediates interaction with SKI and  
Keyword
 Activator; Alternative promoter usage; Alternative splicing; Chromosomal rearrangement; Complete proteome; Differentiation; DNA-binding; Metal-binding; Nucleus; Polymorphism; Reference proteome; Repeat; Repressor; Transcription; Transcription regulation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1276 AA 
Protein Sequence
MRSKARARKL AKSDGDVVNN MYEPNRDLLA SHSAEDEAED SAMSPIPVGP PSPFPTSEDF 60
TPKEGSPYEA PVYIPEDIPI PADFELRESS IPGAGLGVWA KRKMEAGERL GPCVVVPRAA 120
AKETDFGWEQ ILTDVEVSPQ EGCITKISED LGSEKFCVDA NQAGAGSWLK YIRVACSCDD 180
QNLTMCQISE QIYYKVIKDI EPGEELLVHV KEGVYPLGTV PPGLDEEPTF RCDECDELFQ 240
SKLDLRRHKK YTCGSVGAAL YEGLAEELKP EGLGGGSGQA HECKDCERMF PNKYSLEQHM 300
VIHTEEREYK CDQCPKAFNW KSNLIRHQMS HDSGKRFECE NCVKVFTDPS NLQRHIRSQH 360
VGARAHACPD CGKTFATSSG LKQHKHIHST VKPFICEVCH KSYTQFSNLC RHKRMHADCR 420
TQIKCKDCGQ MFSTTSSLNK HRRFCEGKNH YTPGGIFAPG LPLTPSPMMD KAKPSPSLNH 480
ASLGFNEYFP SRPHPGSLPF STAPPTFPAL TPGFPGIFPP SLYPRPPLLP PTSLLKSPLN 540
HTQDAKLPSP LGNPALPLVS AVSNSSQGTT AAAGPEEKFE SRLEDSCVEK LKTRSSDMSD 600
GSDFEDVNTT TGTDLDTTTG TGSDLDSDVD SDPDKDKGKG KSAEGQPKFG GGLAPPGAPN 660
SVAEVPVFYS QHSFFPPPDE QLLTATGAAG DSIKAIASIA EKYFGPGFMG MQEKKLGSLP 720
YHSAFPFQFL PNFPHSLYPF TDRALAHNLL VKAEPKSPRD ALKVGGPSAE CPFDLTTKPK 780
DVKPILPMPK GPSAPASGEE QPLDLSIGSR ARASQNGGGR EPRKNHVYGE RKLGAGEGLP 840
QVCPARMPQQ PPLHYAKPSP FFMDPIYSRV EKRKVTDPVG ALKEKYLRPS PLLFHPQMSA 900
IETMTEKLES FAAMKADSGS SLQPLPHHPF NFRSPPPTLS DPILRKGKER YTCRYCGKIF 960
PRSANLTRHL RTHTGEQPYR CKYCDRSFSI SSNLQRHVRN IHNKEKPFKC HLCNRCFGQQ 1020
TNLDRHLKKH EHENAPVSQH PGVLTNHLGT SASSPTSESD NHALLDEKED SYFSEIRNFI 1080
ANSEMNQAST RTEKRADMQI VDGSAQCPGL ASEKQEDVEE EDDDDLEEDD EDSLAGKSQD 1140
DTVSPAPEPQ AAYEDEEDEE PAASLAVGFD HTRRCAEDHE GGLLALEPMP TFGKGLDLRR 1200
AAEEAFEVKD VLNSTLDSEA LKHTLCRQAK NQAYAMMLSL SEDTPLHTPS QGSLDAWLKV 1260
TGATSESGAF HPINHL 1276 
Gene Ontology
 GO:0017053; C:transcriptional repressor complex; ISS:UniProtKB.
 GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
 GO:0003713; F:transcription coactivator activity; ISS:UniProtKB.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0050873; P:brown fat cell differentiation; ISS:UniProtKB.
 GO:0030853; P:negative regulation of granulocyte differentiation; IDA:UniProtKB.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IDA:UniProtKB.
 GO:0030512; P:negative regulation of transforming growth factor beta receptor signaling pathway; IDA:UniProtKB.
 GO:0022008; P:neurogenesis; IEA:Compara.
 GO:0060021; P:palate development; IEA:Compara.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IDA:UniProtKB.
 GO:0043457; P:regulation of cellular respiration; ISS:UniProtKB.
 GO:0035019; P:somatic stem cell maintenance; IEA:Compara.
 GO:0043586; P:tongue development; IEA:Compara.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0050872; P:white fat cell differentiation; IEA:Compara. 
Interpro
 IPR001214; SET_dom.
 IPR007087; Znf_C2H2.
 IPR015880; Znf_C2H2-like.
 IPR013087; Znf_C2H2/integrase_DNA-bd. 
Pfam
 PF00096; zf-C2H2 
SMART
 SM00317; SET
 SM00355; ZnF_C2H2 
PROSITE
 PS50280; SET
 PS00028; ZINC_FINGER_C2H2_1
 PS50157; ZINC_FINGER_C2H2_2 
PRINTS