CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006275
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Kinesin-1 heavy chain 
Protein Synonyms/Alias
 Conventional kinesin heavy chain; Ubiquitous kinesin heavy chain; UKHC 
Gene Name
 KIF5B 
Gene Synonyms/Alias
 KNS; KNS1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
10DLAECNIKVMCRFRPacetylation[1]
10DLAECNIKVMCRFRPubiquitination[1, 2]
28SEVNRGDKYIAKFQGacetylation[3]
222ENTQTEQKLSGKLYLubiquitination[4]
226TEQKLSGKLYLVDLAubiquitination[2, 5]
240AGSEKVSKTGAEGAVubiquitination[2]
252GAVLDEAKNINKSLSubiquitination[4]
256DEAKNINKSLSALGNubiquitination[2]
382PIDEQFDKEKANLEAacetylation[6]
512KSQEVEDKTKEYELLacetylation[1, 7]
761LKATDQEKSRKLHELacetylation[8]
764TDQEKSRKLHELTVMacetylation[8]
783EQARQDLKGLEETVAubiquitination[4, 9]
801QTLHNLRKLFVQDLAubiquitination[2]
829TGGSAAQKQKISFLEubiquitination[2]
844NNLEQLTKVHKQLVRubiquitination[2]
885ESALKEAKENASRDRubiquitination[10]
922GHSAQIAKPIRPGQHubiquitination[5]
Reference
 [1] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [6] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [7] Monoclonal antibody cocktail as an enrichment tool for acetylome analysis.
 Shaw PG, Chaerkady R, Zhang Z, Davidson NE, Pandey A.
 Anal Chem. 2011 May 15;83(10):3623-6. [PMID: 21466224]
 [8] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [9] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [10] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
 Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes (By similarity). 
Sequence Annotation
 DOMAIN 2 328 Kinesin-motor.
 NP_BIND 85 92 ATP.
 REGION 915 963 Globular.
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 933 933 Phosphoserine.  
Keyword
 3D-structure; Acetylation; ATP-binding; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Microtubule; Motor protein; Nucleotide-binding; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 963 AA 
Protein Sequence
MADLAECNIK VMCRFRPLNE SEVNRGDKYI AKFQGEDTVV IASKPYAFDR VFQSSTSQEQ 60
VYNDCAKKIV KDVLEGYNGT IFAYGQTSSG KTHTMEGKLH DPEGMGIIPR IVQDIFNYIY 120
SMDENLEFHI KVSYFEIYLD KIRDLLDVSK TNLSVHEDKN RVPYVKGCTE RFVCSPDEVM 180
DTIDEGKSNR HVAVTNMNEH SSRSHSIFLI NVKQENTQTE QKLSGKLYLV DLAGSEKVSK 240
TGAEGAVLDE AKNINKSLSA LGNVISALAE GSTYVPYRDS KMTRILQDSL GGNCRTTIVI 300
CCSPSSYNES ETKSTLLFGQ RAKTIKNTVC VNVELTAEQW KKKYEKEKEK NKILRNTIQW 360
LENELNRWRN GETVPIDEQF DKEKANLEAF TVDKDITLTN DKPATAIGVI GNFTDAERRK 420
CEEEIAKLYK QLDDKDEEIN QQSQLVEKLK TQMLDQEELL ASTRRDQDNM QAELNRLQAE 480
NDASKEEVKE VLQALEELAV NYDQKSQEVE DKTKEYELLS DELNQKSATL ASIDAELQKL 540
KEMTNHQKKR AAEMMASLLK DLAEIGIAVG NNDVKQPEGT GMIDEEFTVA RLYISKMKSE 600
VKTMVKRCKQ LESTQTESNK KMEENEKELA ACQLRISQHE AKIKSLTEYL QNVEQKKRQL 660
EESVDALSEE LVQLRAQEKV HEMEKEHLNK VQTANEVKQA VEQQIQSHRE THQKQISSLR 720
DEVEAKAKLI TDLQDQNQKM MLEQERLRVE HEKLKATDQE KSRKLHELTV MQDRREQARQ 780
DLKGLEETVA KELQTLHNLR KLFVQDLATR VKKSAEIDSD DTGGSAAQKQ KISFLENNLE 840
QLTKVHKQLV RDNADLRCEL PKLEKRLRAT AERVKALESA LKEAKENASR DRKRYQQEVD 900
RIKEAVRSKN MARRGHSAQI AKPIRPGQHP AASPTHPSAI RGGGAFVQNS QPVAVRGGGG 960
KQV 963 
Gene Ontology
 GO:0035253; C:ciliary rootlet; IEA:Compara.
 GO:0005871; C:kinesin complex; TAS:ProtInc.
 GO:0005874; C:microtubule; IEA:UniProtKB-KW.
 GO:0043005; C:neuron projection; IEA:Compara.
 GO:0048471; C:perinuclear region of cytoplasm; ISS:HGNC.
 GO:0031982; C:vesicle; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0008017; F:microtubule binding; ISS:HGNC.
 GO:0003777; F:microtubule motor activity; ISS:HGNC.
 GO:0044267; P:cellular protein metabolic process; TAS:Reactome.
 GO:0007028; P:cytoplasm organization; IEA:Compara.
 GO:0090004; P:positive regulation of establishment of protein localization to plasma membrane; IDA:BHF-UCL.
 GO:0043268; P:positive regulation of potassium ion transport; IDA:BHF-UCL.
 GO:0042391; P:regulation of membrane potential; IDA:BHF-UCL.
 GO:0035617; P:stress granule disassembly; ISS:BHF-UCL.
 GO:0047496; P:vesicle transport along microtubule; ISS:HGNC. 
Interpro
 IPR019821; Kinesin_motor_CS.
 IPR001752; Kinesin_motor_dom.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00225; Kinesin 
SMART
 SM00129; KISc 
PROSITE
 PS00411; KINESIN_MOTOR_DOMAIN1
 PS50067; KINESIN_MOTOR_DOMAIN2 
PRINTS
 PR00380; KINESINHEAVY.