UniProt Accession

 OPSD_BOVIN; P02699 

Genbank Protein ID

 K00506; K00502; K00503; K00504; K00505; M21606 

Genbank Nucleotide ID

 AAA30674.1; AAA30674.1; AAA30674.1; AAA30674.1; AAA30674.1; AAA30675.1 

Protein Name

 Rhodopsin 

Protein Synonyms/Alias

  

Gene Name

 RHO 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Bos taurus (Bovine) 

NCBI Taxa ID

 9913 

Lysine Modification

Position	Peptide	Type	References
16	FYVPFSNKTGVVRSP	acetylation	[1]
66	LYVTVQHKKLRTPLN	acetylation	[1]
67	YVTVQHKKLRTPLNY	acetylation	[1]
141	ERYVVVCKPMSNFRF	acetylation	[1]
311	VIYIMMNKQFRNCMV	acetylation	[1]
325	VTTLCCGKNPLGDDE	acetylation	[1]
339	EASTTVSKTETSQVA	acetylation	[1]

Reference

 [1] Probing rhodopsin-transducin interactions by surface modification and mass spectrometry.
 Wang X, Kim SH, Ablonczy Z, Crouch RK, Knapp DR.
 Biochemistry. 2004 Sep 7;43(35):11153-62. [PMID: 15366925] 

Functional Description

 Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal (By similarity). 

Sequence Annotation

 REGION 113 125 Retinal chromophore binding.
 REGION 207 212 Retinal chromophore binding.
 MOTIF 134 137 'Ionic lock' involved in activated form
 METAL 201 201 Zinc.
 METAL 279 279 Zinc.
 BINDING 265 265 Retinal chromophore.
 BINDING 296 296 Retinal chromophore (covalent).
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 296 296 N6-(retinylidene)lysine.
 MOD_RES 334 334 Phosphoserine (By similarity).
 MOD_RES 335 335 Phosphothreonine.
 MOD_RES 336 336 Phosphothreonine.
 MOD_RES 338 338 Phosphoserine (By similarity).
 MOD_RES 340 340 Phosphothreonine.
 MOD_RES 342 342 Phosphothreonine.
 MOD_RES 343 343 Phosphoserine; by RK and GRK7.
 LIPID 322 322 S-palmitoyl cysteine.
 LIPID 323 323 S-palmitoyl cysteine.
 CARBOHYD 2 2 N-linked (GlcNAc...).
 CARBOHYD 15 15 N-linked (GlcNAc...).
 DISULFID 110 187  

Keyword

 3D-structure; Acetylation; Chromophore; Complete proteome; Direct protein sequencing; Disulfide bond; G-protein coupled receptor; Glycoprotein; Lipoprotein; Membrane; Metal-binding; Palmitate; Phosphoprotein; Photoreceptor protein; Receptor; Reference proteome; Retinal protein; Sensory transduction; Transducer; Transmembrane; Transmembrane helix; Vision; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 348 AA 

Protein Sequence

Gene Ontology

 GO:0005794; C:Golgi apparatus; IEA:Compara.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0060342; C:photoreceptor inner segment membrane; ISS:UniProtKB.
 GO:0042622; C:photoreceptor outer segment membrane; ISS:UniProtKB.
 GO:0005886; C:plasma membrane; ISS:UniProtKB.
 GO:0004930; F:G-protein coupled receptor activity; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0009881; F:photoreceptor activity; IEA:UniProtKB-KW.
 GO:0071482; P:cellular response to light stimulus; IEA:Compara.
 GO:0007602; P:phototransduction; IEA:UniProtKB-KW.
 GO:0006468; P:protein phosphorylation; IEA:Compara.
 GO:0018298; P:protein-chromophore linkage; IEA:UniProtKB-KW.
 GO:0060041; P:retina development in camera-type eye; IEA:Compara.
 GO:0007601; P:visual perception; IEA:UniProtKB-KW. 

Interpro

 IPR000276; GPCR_Rhodpsn.
 IPR017452; GPCR_Rhodpsn_7TM.
 IPR001760; Opsin.
 IPR027430; Retinal_BS.
 IPR000732; Rhodopsin.
 IPR019477; Rhodopsin_N. 

Pfam

 PF00001; 7tm_1
 PF10413; Rhodopsin_N 

SMART

  

PROSITE

 PS00237; G_PROTEIN_RECEP_F1_1
 PS50262; G_PROTEIN_RECEP_F1_2
 PS00238; OPSIN 

PRINTS

 PR00237; GPCRRHODOPSN.
 PR00238; OPSIN.
 PR00579; RHODOPSIN.