CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-009271
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein S100-A10 
Protein Synonyms/Alias
 Calpactin I light chain; Calpactin-1 light chain; Cellular ligand of annexin II; S100 calcium-binding protein A10; p10 protein; p11 
Gene Name
 S100A10 
Gene Synonyms/Alias
 ANX2LG; CAL1L; CLP11 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
18TMMFTFHKFAGDKGYubiquitination[1, 2]
23FHKFAGDKGYLTKEDacetylation[3, 4, 5]
23FHKFAGDKGYLTKEDubiquitination[6]
28GDKGYLTKEDLRVLMacetylation[3, 4, 5]
28GDKGYLTKEDLRVLMubiquitination[7]
37DLRVLMEKEFPGFLEacetylation[3, 4, 5]
37DLRVLMEKEFPGFLEubiquitination[1, 2, 6, 7, 8, 9, 10, 11]
47PGFLENQKDPLAVDKubiquitination[1, 2, 7, 8, 10, 12]
54KDPLAVDKIMKDLDQacetylation[3, 4]
54KDPLAVDKIMKDLDQubiquitination[1, 2]
57LAVDKIMKDLDQCRDacetylation[3, 4, 5]
57LAVDKIMKDLDQCRDubiquitination[1, 2]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [4] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [5] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [6] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [7] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [8] Global analysis of lysine ubiquitination by ubiquitin remnant immunoaffinity profiling.
 Xu G, Paige JS, Jaffrey SR.
 Nat Biotechnol. 2010 Aug;28(8):868-73. [PMID: 20639865]
 [9] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [10] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [11] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [12] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048
Functional Description
 Because S100A10 induces the dimerization of ANXA2/p36, it may function as a regulator of protein phosphorylation in that the ANXA2 monomer is the preferred target (in vitro) of tyrosine- specific kinase. 
Sequence Annotation
 REGION 60 71 Ancestral calcium site.
 MOD_RES 23 23 N6-acetyllysine.
 MOD_RES 28 28 N6-acetyllysine.
 MOD_RES 37 37 N6-acetyllysine.
 MOD_RES 54 54 N6-acetyllysine.
 MOD_RES 57 57 N6-acetyllysine.  
Keyword
 3D-structure; Acetylation; Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 97 AA 
Protein Sequence
MPSQMEHAME TMMFTFHKFA GDKGYLTKED LRVLMEKEFP GFLENQKDPL AVDKIMKDLD 60
QCRDGKVGFQ SFFSLIAGLT IACNDYFVVH MKQKGKK 97 
Gene Ontology
 GO:0019897; C:extrinsic to plasma membrane; IEA:Compara.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0005102; F:receptor binding; NAS:ProtInc.
 GO:0071229; P:cellular response to acid; IEA:Compara.
 GO:0051099; P:positive regulation of binding; IEA:Compara.
 GO:0007165; P:signal transduction; NAS:ProtInc. 
Interpro
 IPR011992; EF-hand-like_dom.
 IPR001751; S100/CaBP-9k_CS.
 IPR013787; S100_Ca-bd_sub. 
Pfam
 PF01023; S_100 
SMART
  
PROSITE
 PS00303; S100_CABP 
PRINTS