CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019272
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Centrosomal protein of 95 kDa 
Protein Synonyms/Alias
 Coiled-coil domain-containing protein 45 
Gene Name
 CEP95 
Gene Synonyms/Alias
 CCDC45; CEP45 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
130SETEQYFKESDRGERubiquitination[1]
145LEEPESTKESKSSWKubiquitination[2, 3]
208PNEMLSKKALASPSSubiquitination[1]
230YPPSVLSKSRTSFVEubiquitination[1]
314DGLFLISKLPKGSKWubiquitination[1]
320SKLPKGSKWEVYPAQubiquitination[1, 2, 4, 5]
374ELHDVSEKLSQRLSEubiquitination[1, 2]
387SELDWMLKSALGDRIubiquitination[1, 2, 6]
496ERELRRHKVQENIGPubiquitination[1]
524YRGEAVRKGTPECSQubiquitination[1]
588PTLSKMWKQQIAQVEubiquitination[1]
598IAQVEQLKKEACRENubiquitination[1, 2]
610RENRSKKKLQDEIEEubiquitination[1]
630DLLTTLVKKEYEHNKubiquitination[1]
643NKRLQDFKDCIRRQRubiquitination[1]
668QQIVRARKYYDDYRVubiquitination[1]
680YRVQLCAKMMRMRTRubiquitination[1]
694REEMIFKKLFEEGLNubiquitination[1]
704EEGLNIQKQRLRDLRubiquitination[2, 6]
755SQEHQELKAREKSQAubiquitination[2]
817LASFQYSKSPSL***ubiquitination[1, 6]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724
Functional Description
  
Sequence Annotation
 MOD_RES 449 449 Phosphoserine.
 MOD_RES 451 451 Phosphoserine.
 MOD_RES 453 453 Phosphoserine.  
Keyword
 Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 821 AA 
Protein Sequence
MAGSDAEWVT IANNLLFKCH IHLRIHELQD CDANVFIALY QSILGEKVPD LIVIPRSQED 60
DAHNVQAVID SLALDYLQVS LSHITGENIV KGDKESIKNL LEIFDGLLEY LTERISETSH 120
EKSETEQYFK ESDRGERLEE PESTKESKSS WKRVSFGRCS LSSEMLGPSW DGDEAESTGE 180
IIRLGDTAHT FSLRSNGAQC PNEMLSKKAL ASPSSKSHED MLYPPSVLSK SRTSFVEDTE 240
TLSVSGIPNA RKLGEPIRAA IPLHPPYHPS EPRAPCPIGK EYLHSSHCSP AVNSTGEHTE 300
FSGDLDDGLF LISKLPKGSK WEVYPAQVQG PRTRKPPKGK RNENRATASS CNSPFPQRPR 360
KRLTEQELHD VSEKLSQRLS ELDWMLKSAL GDRIKEKTDH KEENTGNEEV EDGTEETLSQ 420
HSDGIVEYGP KKSRPGLSMR RKPPYRSHSL SPSPVNKHKQ FHLERKRQRK PRETDVRQFQ 480
AQAFTEAFER ELRRHKVQEN IGPLRIHEKE EETEKIYRGE AVRKGTPECS QPWKIYSRKT 540
TTQSLRGGLP KPNKAVPMKV SEHSLLPLML EQFPFLYVSG PTLSKMWKQQ IAQVEQLKKE 600
ACRENRSKKK LQDEIEEALR RHDLLTTLVK KEYEHNKRLQ DFKDCIRRQR LTQSKIKENR 660
QQIVRARKYY DDYRVQLCAK MMRMRTREEM IFKKLFEEGL NIQKQRLRDL RNYAKEKRDE 720
QRRRHQDELD SMENYYKDQF SLLAEAISQE HQELKAREKS QAQTLHKVKR ELRSKMEKEI 780
QQLQDMITQN DDDVFFRELE AERFRSRLQL ASFQYSKSPS L 821 
Gene Ontology
 GO:0005813; C:centrosome; IDA:UniProtKB.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
 GO:0000922; C:spindle pole; IDA:UniProtKB. 
Interpro
 IPR026619; CEP95.
 IPR001715; CH-domain. 
Pfam
  
SMART
  
PROSITE
  
PRINTS