CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022792
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein ROS1 
Protein Synonyms/Alias
 DEMETER-like protein 1; Repressor of silencing 1 
Gene Name
 ROS1 
Gene Synonyms/Alias
 DML1; At2g36490; F1O11.12 
Created Date
 July 27, 2013 
Organism
 Arabidopsis thaliana (Mouse-ear cress) 
NCBI Taxa ID
 3702 
Lysine Modification
Position
Peptide
Type
References
901KEVAETIKSRGMNHKubiquitination[1]
Reference
 [1] Multidimensional protein identification technology (MudPIT) analysis of ubiquitinated proteins in plants.
 Maor R, Jones A, Nühse TS, Studholme DJ, Peck SC, Shirasu K.
 Mol Cell Proteomics. 2007 Apr;6(4):601-10. [PMID: 17272265] 
Functional Description
 Prevents DNA hypermethylation, specifically in the promoter of otherwise silenced loci. May be involved in DNA repair through its nicking activity on methylated DNA. Catalyzes the release of 5-methylcytosine (5-meC) from DNA by a glycosylase/lyase mechanism. Binds with similar affinity to both methylated and non-methylated DNA. Highly distributive behavior on DNA substrates containing multiple 5-meC residues. Involved with Pol IV in the remodeling of the 5S rDNA chromatin via DNA methylation modifications during the first days of development post-germination. 
Sequence Annotation
 REGION 528 626 DEMETER.
 METAL 1038 1038 Iron-sulfur (4Fe-4S) (By similarity).
 METAL 1045 1045 Iron-sulfur (4Fe-4S) (By similarity).
 METAL 1048 1048 Iron-sulfur (4Fe-4S) (By similarity).
 METAL 1054 1054 Iron-sulfur (4Fe-4S) (By similarity).
 CROSSLNK 901 901 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 4Fe-4S; Activator; Complete proteome; DNA-binding; Hydrolase; Iron; Iron-sulfur; Isopeptide bond; Metal-binding; Nucleus; Reference proteome; Transcription; Transcription regulation; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1393 AA 
Protein Sequence
MEKQRREESS FQQPPWIPQT PMKPFSPICP YTVEDQYHSS QLEERRFVGN KDMSGLDHLS 60
FGDLLALANT ASLIFSGQTP IPTRNTEVMQ KGTEEVESLS SVSNNVAEQI LKTPEKPKRK 120
KHRPKVRREA KPKREPKPRA PRKSVVTDGQ ESKTPKRKYV RKKVEVSKDQ DATPVESSAA 180
VETSTRPKRL CRRVLDFEAE NGENQTNGDI REAGEMESAL QEKQLDSGNQ ELKDCLLSAP 240
STPKRKRSQG KRKGVQPKKN GSNLEEVDIS MAQAAKRRQG PTCCDMNLSG IQYDEQCDYQ 300
KMHWLYSPNL QQGGMRYDAI CSKVFSGQQH NYVSAFHATC YSSTSQLSAN RVLTVEERRE 360
GIFQGRQESE LNVLSDKIDT PIKKKTTGHA RFRNLSSMNK LVEVPEHLTS GYCSKPQQNN 420
KILVDTRVTV SKKKPTKSEK SQTKQKNLLP NLCRFPPSFT GLSPDELWKR RNSIETISEL 480
LRLLDINREH SETALVPYTM NSQIVLFGGG AGAIVPVTPV KKPRPRPKVD LDDETDRVWK 540
LLLENINSEG VDGSDEQKAK WWEEERNVFR GRADSFIARM HLVQGDRRFT PWKGSVVDSV 600
VGVFLTQNVS DHLSSSAFMS LASQFPVPFV PSSNFDAGTS SMPSIQITYL DSEETMSSPP 660
DHNHSSVTLK NTQPDEEKDY VPSNETSRSS SEIAISAHES VDKTTDSKEY VDSDRKGSSV 720
EVDKTDEKCR VLNLFPSEDS ALTCQHSMVS DAPQNTERAG SSSEIDLEGE YRTSFMKLLQ 780
GVQVSLEDSN QVSPNMSPGD CSSEIKGFQS MKEPTKSSVD SSEPGCCSQQ DGDVLSCQKP 840
TLKEKGKKVL KEEKKAFDWD CLRREAQARA GIREKTRSTM DTVDWKAIRA ADVKEVAETI 900
KSRGMNHKLA ERIQGFLDRL VNDHGSIDLE WLRDVPPDKA KEYLLSFNGL GLKSVECVRL 960
LTLHHLAFPV DTNVGRIAVR LGWVPLQPLP ESLQLHLLEM YPMLESIQKY LWPRLCKLDQ 1020
KTLYELHYQM ITFGKVFCTK SKPNCNACPM KGECRHFASA FASARLALPS TEKGMGTPDK 1080
NPLPLHLPEP FQREQGSEVV QHSEPAKKVT CCEPIIEEPA SPEPETAEVS IADIEEAFFE 1140
DPEEIPTIRL NMDAFTSNLK KIMEHNKELQ DGNMSSALVA LTAETASLPM PKLKNISQLR 1200
TEHRVYELPD EHPLLAQLEK REPDDPCSYL LAIWTPGETA DSIQPSVSTC IFQANGMLCD 1260
EETCFSCNSI KETRSQIVRG TILIPCRTAM RGSFPLNGTY FQVNEVFADH ASSLNPINVP 1320
RELIWELPRR TVYFGTSVPT IFKGLSTEKI QACFWKGYVC VRGFDRKTRG PKPLIARLHF 1380
PASKLKGQQA NLA 1393 
Gene Ontology
 GO:0005634; C:nucleus; IDA:TAIR.
 GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0019104; F:DNA N-glycosylase activity; IDA:TAIR.
 GO:0003906; F:DNA-(apurinic or apyrimidinic site) lyase activity; IDA:TAIR.
 GO:0004519; F:endonuclease activity; IEA:InterPro.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0006284; P:base-excision repair; IEA:InterPro.
 GO:0006342; P:chromatin silencing; IMP:TAIR.
 GO:0080111; P:DNA demethylation; IMP:TAIR.
 GO:0006306; P:DNA methylation; IDA:TAIR.
 GO:0006281; P:DNA repair; IMP:TAIR.
 GO:0031936; P:negative regulation of chromatin silencing; IMP:TAIR.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR011257; DNA_glycosylase.
 IPR003651; Endouclease3_FeS-loop_motif.
 IPR003265; HhH-GPD_domain.
 IPR023170; HTH_base_excis_C. 
Pfam
 PF00730; HhH-GPD 
SMART
 SM00478; ENDO3c
 SM00525; FES 
PROSITE
 PS00764; ENDONUCLEASE_III_1 
PRINTS