UniProt Accession

 STK24_HUMAN; Q9Y6E0; O14840; Q5JV92 

Genbank Protein ID

 AF024636; AF083420; AL356423; AL137249; AL137249; AL356423; CH471085; BC035578 

Genbank Nucleotide ID

 AAB82560.1; AAD42039.1; CAI13114.1; CAI13114.1; CAI39453.1; CAI39453.1; EAX08986.1; AAH35578.1 

Protein Name

 Serine/threonine-protein kinase 24 

Protein Synonyms/Alias

 Mammalian STE20-like protein kinase 3; MST-3; STE20-like kinase MST3; Serine/threonine-protein kinase 24 36 kDa subunit; Mammalian STE20-like protein kinase 3 N-terminal; MST3/N; Serine/threonine-protein kinase 24 12 kDa subunit; Mammalian STE20-like protein kinase 3 C-terminal; MST3/C 

Gene Name

 STK24 

Gene Synonyms/Alias

 MST3; STK3 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
52	GSFGEVFKGIDNRTQ	ubiquitination	[1, 2]
152	DYLHSEKKIHRDIKA	ubiquitination	[2]
187	QLTDTQIKRNTFVGT	ubiquitination	[2]
245	KVLFLIPKNNPPTLE	ubiquitination	[1]
292	FILRNAKKTSYLTEL	ubiquitination	[1, 2]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Serine/threonine-protein kinase that acts on both serine and threonine residues and promotes apoptosis in response to stress stimuli and caspase activation. Mediates oxidative-stress- induced cell death by modulating phosphorylation of JNK1-JNK2 (MAPK8 and MAPK9), p38 (MAPK11, MAPK12, MAPK13 and MAPK14) during oxidative stress. Plays a role in a staurosporine-induced caspase- independent apoptotic pathway by regulating the nuclear translocation of AIFM1 and ENDOG and the DNase activity associated with ENDOG. Phosphorylates STK38L on 'Thr-442' and stimulates its kinase activity. Regulates cellular migration with alteration of PTPN12 activity and PXN phosphorylation: phosphorylates PTPN12 and inhibits its activity and may regulate PXN phosphorylation through PTPN12. May act as a key regulator of axon regeneration in the optic nerve and radial nerve. 

Sequence Annotation

 DOMAIN 36 286 Protein kinase.
 NP_BIND 42 50 ATP.
 NP_BIND 112 114 ATP.
 REGION 335 386 Nuclear export signal (NES).
 MOTIF 278 292 Bipartite nuclear localization signal.
 ACT_SITE 156 156 Proton acceptor (By similarity).
 METAL 161 161 Magnesium.
 METAL 174 174 Magnesium.
 BINDING 65 65 ATP.
 MOD_RES 18 18 Phosphothreonine; by PKA.
 MOD_RES 190 190 Phosphothreonine; by autocatalysis.
 MOD_RES 320 320 Phosphoserine.  

Keyword

 3D-structure; Acetylation; Alternative splicing; Apoptosis; ATP-binding; Complete proteome; Cytoplasm; Kinase; Magnesium; Membrane; Metal-binding; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 443 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004674; F:protein serine/threonine kinase activity; IDA:UniProtKB.
 GO:0006921; P:cellular component disassembly involved in execution phase of apoptosis; TAS:Reactome.
 GO:0008631; P:intrinsic apoptotic signaling pathway in response to oxidative stress; IMP:UniProtKB.
 GO:0030336; P:negative regulation of cell migration; IMP:UniProtKB.
 GO:0046777; P:protein autophosphorylation; IDA:UniProtKB.
 GO:0048679; P:regulation of axon regeneration; IMP:UniProtKB. 

Interpro

 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom. 

Pfam

 PF00069; Pkinase 

SMART

 SM00220; S_TKc 

PROSITE

 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 

PRINTS