CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-003197
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Aldehyde-alcohol dehydrogenase 
Protein Synonyms/Alias
 Alcohol dehydrogenase; ADH; Acetaldehyde dehydrogenase [acetylating]; ACDH; Pyruvate-formate-lyase deactivase; PFL deactivase 
Gene Name
 adhE 
Gene Synonyms/Alias
 ana; b1241; JW1228 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
33FTQEQVDKIFRAAALacetylation[1, 2]
51DARIPLAKMAVAESGacetylation[1]
65GMGIVEDKVIKNHFAacetylation[2]
68IVEDKVIKNHFASEYacetylation[1, 2]
81EYIYNAYKDEKTCGVacetylation[2]
129FKSLISLKTRNAIIFacetylation[2]
148RAKDATNKAADIVLQacetylation[1, 2]
200TGGPGMVKAAYSSGKacetylation[3]
207KAAYSSGKPAIGVGAacetylation[2]
228IDETADIKRAVASVLacetylation[1, 2]
277GGYLLQGKELKAVQDacetylation[1, 2]
280LLQGKELKAVQDVILacetylation[1, 2]
288AVQDVILKNGALNAAacetylation[1, 2]
303IVGQPAYKIAELAGFacetylation[2]
347TLAMYRAKDFEDAVEacetylation[2]
355DFEDAVEKAEKLVAMacetylation[2]
358DAVEKAEKLVAMGGIacetylation[1, 2, 4]
388RVSYFGQKMKTARILacetylation[1, 2]
436ISENVGPKHLINKKTacetylation[1, 2]
441GPKHLINKKTVAKRAacetylation[2]
455AENMLWHKLPKSIYFacetylation[2]
458MLWHKLPKSIYFRRGacetylation[1, 2]
480EVITDGHKRALIVTDacetylation[2]
536AELANSFKPDVIIALacetylation[2]
582DIRKRIYKFPKMGVKacetylation[2]
683LQALKLLKEYLPASYacetylation[1, 2]
695ASYHEGSKNPVARERacetylation[1, 2]
759NANDNPTKQTAFSQYacetylation[2]
794PGDRTAAKIEKLLAWacetylation[2]
797RTAAKIEKLLAWLETacetylation[1, 2]
813KAELGIPKSIREAGVacetylation[1, 2]
831DFLANVDKLSEDAFDacetylation[2]
875VEGETAAKKEAAPAKacetylation[2]
Reference
 [1] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111]
 [2] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [3] The diversity of lysine-acetylated proteins in Escherichia coli.
 Yu BJ, Kim JA, Moon JH, Ryu SE, Pan JG.
 J Microbiol Biotechnol. 2008 Sep;18(9):1529-36. [PMID: 18852508]
 [4] Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli.
 Zhang J, Sprung R, Pei J, Tan X, Kim S, Zhu H, Liu CF, Grishin NV, Zhao Y.
 Mol Cell Proteomics. 2009 Feb;8(2):215-25. [PMID: 18723842] 
Functional Description
 This enzyme has three activities: ADH, ACDH, and PFL- deactivase. In aerobic conditions it acts as a hydrogen peroxide scavenger. The PFL deactivase activity catalyzes the quenching of the pyruvate-formate-lyase catalyst in an iron, NAD, and CoA dependent reaction. 
Sequence Annotation
 NP_BIND 422 427 NAD (Potential).
 ACT_SITE 246 246 By similarity.
 MOD_RES 358 358 N6-acetyllysine.  
Keyword
 Acetylation; Complete proteome; Direct protein sequencing; Iron; Multifunctional enzyme; NAD; Oxidoreductase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 891 AA 
Protein Sequence
MAVTNVAELN ALVERVKKAQ REYASFTQEQ VDKIFRAAAL AAADARIPLA KMAVAESGMG 60
IVEDKVIKNH FASEYIYNAY KDEKTCGVLS EDDTFGTITI AEPIGIICGI VPTTNPTSTA 120
IFKSLISLKT RNAIIFSPHP RAKDATNKAA DIVLQAAIAA GAPKDLIGWI DQPSVELSNA 180
LMHHPDINLI LATGGPGMVK AAYSSGKPAI GVGAGNTPVV IDETADIKRA VASVLMSKTF 240
DNGVICASEQ SVVVVDSVYD AVRERFATHG GYLLQGKELK AVQDVILKNG ALNAAIVGQP 300
AYKIAELAGF SVPENTKILI GEVTVVDESE PFAHEKLSPT LAMYRAKDFE DAVEKAEKLV 360
AMGGIGHTSC LYTDQDNQPA RVSYFGQKMK TARILINTPA SQGGIGDLYN FKLAPSLTLG 420
CGSWGGNSIS ENVGPKHLIN KKTVAKRAEN MLWHKLPKSI YFRRGSLPIA LDEVITDGHK 480
RALIVTDRFL FNNGYADQIT SVLKAAGVET EVFFEVEADP TLSIVRKGAE LANSFKPDVI 540
IALGGGSPMD AAKIMWVMYE HPETHFEELA LRFMDIRKRI YKFPKMGVKA KMIAVTTTSG 600
TGSEVTPFAV VTDDATGQKY PLADYALTPD MAIVDANLVM DMPKSLCAFG GLDAVTHAME 660
AYVSVLASEF SDGQALQALK LLKEYLPASY HEGSKNPVAR ERVHSAATIA GIAFANAFLG 720
VCHSMAHKLG SQFHIPHGLA NALLICNVIR YNANDNPTKQ TAFSQYDRPQ ARRRYAEIAD 780
HLGLSAPGDR TAAKIEKLLA WLETLKAELG IPKSIREAGV QEADFLANVD KLSEDAFDDQ 840
CTGANPRYPL ISELKQILLD TYYGRDYVEG ETAAKKEAAP AKAEKKAKKS A 891 
Gene Ontology
 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0016020; C:membrane; IDA:UniProtKB.
 GO:0008774; F:acetaldehyde dehydrogenase (acetylating) activity; IMP:EcoliWiki.
 GO:0004022; F:alcohol dehydrogenase (NAD) activity; IMP:EcoliWiki.
 GO:0046872; F:metal ion binding; IEA:InterPro.
 GO:0015976; P:carbon utilization; IEA:InterPro.
 GO:0006115; P:ethanol biosynthetic process; IDA:EcoliWiki. 
Interpro
 IPR001670; ADH_Fe.
 IPR018211; ADH_Fe_CS.
 IPR016161; Ald_DH/histidinol_DH.
 IPR016163; Ald_DH_C.
 IPR016162; Ald_DH_N.
 IPR015590; Aldehyde_DH_dom.
 IPR012079; Bifunc_Ald/ADH. 
Pfam
 PF00171; Aldedh
 PF00465; Fe-ADH 
SMART
  
PROSITE
 PS00913; ADH_IRON_1
 PS00060; ADH_IRON_2 
PRINTS