UniProt Accession

 MAS5_YEAST; P25491; D6W1B6 

Genbank Protein ID

 X56560; S74758; U12141; Z71340; BK006947 

Genbank Nucleotide ID

 CAA39910.1; AAB20771.1; AAA99647.1; CAA95937.1; DAA10482.1 

Protein Name

 Mitochondrial protein import protein MAS5 

Protein Synonyms/Alias

 Yeast dnaJ protein 1 

Gene Name

 YDJ1 

Gene Synonyms/Alias

 MAS5; YNL064C; N2418; YNL2418C 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
23	TATDVEIKKAYRKCA	acetylation	[1]
23	TATDVEIKKAYRKCA	ubiquitination	[2, 3]
37	ALKYHPDKNPSEEAA	acetylation	[1]
37	ALKYHPDKNPSEEAA	ubiquitination	[3]
46	PSEEAAEKFKEASAA	acetylation	[1]
117	PQRGKDIKHEISASL	acetylation	[1]
129	ASLEELYKGRTAKLA	acetylation	[1]
134	LYKGRTAKLALNKQI	ubiquitination	[3]
139	TAKLALNKQILCKEC	ubiquitination	[3]
144	LNKQILCKECEGRGG	ubiquitination	[3]
158	GKKGAVKKCTSCNGQ	ubiquitination	[3]
198	TGDIIDPKDRCKSCN	ubiquitination	[2, 3]
314	MRKVIEGKGMPIPKY	ubiquitination	[3]
320	GKGMPIPKYGGYGNL	acetylation	[1]
334	LIIKFTIKFPENHFT	acetylation	[1]
348	TSEENLKKLEEILPP	ubiquitination	[3]
363	RIVPAIPKKATVDEC	ubiquitination	[3]
364	IVPAIPKKATVDECV	ubiquitination	[3]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [3] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Probably involved in mitochondrial protein import. Is also required for efficient translocation of pre-pro-alpha-factor. Involved in heme regulation of HAP1, as a component of the high- molecular-weight (HMC) complex. 

Sequence Annotation

 DOMAIN 4 72 J.
 REPEAT 143 150 CXXCXGXG motif.
 REPEAT 159 166 CXXCXGXG motif.
 REPEAT 185 192 CXXCXGXG motif.
 REPEAT 201 208 CXXCXGXG motif.
 ZN_FING 130 213 CR-type.
 REGION 1 172 Necessary for HAP1 repression in the
 REGION 135 137 Substrate binding.
 REGION 215 216 Substrate binding.
 REGION 247 249 Substrate binding.
 METAL 143 143 Zinc 1.
 METAL 146 146 Zinc 1.
 METAL 159 159 Zinc 2.
 METAL 162 162 Zinc 2.
 METAL 185 185 Zinc 2.
 METAL 188 188 Zinc 2.
 METAL 201 201 Zinc 1.
 METAL 204 204 Zinc 1.
 BINDING 116 116 Substrate; via amide nitrogen.
 MOD_RES 406 406 Cysteine methyl ester (Probable).
 LIPID 406 406 S-farnesyl cysteine.  

Keyword

 3D-structure; Chaperone; Complete proteome; Cytoplasm; Lipoprotein; Metal-binding; Methylation; Prenylation; Protein transport; Reference proteome; Repeat; Stress response; Transport; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 409 AA 

Protein Sequence

Gene Ontology

 GO:0048471; C:perinuclear region of cytoplasm; IDA:SGD.
 GO:0072380; C:TRC complex; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:InterPro.
 GO:0001671; F:ATPase activator activity; IDA:SGD.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0051082; F:unfolded protein binding; IDA:SGD.
 GO:0006458; P:'de novo' protein folding; IMP:SGD.
 GO:0030433; P:ER-associated protein catabolic process; IMP:SGD.
 GO:0042026; P:protein refolding; IDA:SGD.
 GO:0045047; P:protein targeting to ER; IMP:SGD.
 GO:0006626; P:protein targeting to mitochondrion; IMP:SGD.
 GO:0009408; P:response to heat; IEA:InterPro. 

Interpro

 IPR012724; DnaJ.
 IPR002939; DnaJ_C.
 IPR001623; DnaJ_domain.
 IPR018253; DnaJ_domain_CS.
 IPR008971; HSP40/DnaJ_pept-bd.
 IPR001305; HSP_DnaJ_Cys-rich_dom. 

Pfam

 PF00226; DnaJ
 PF01556; DnaJ_C
 PF00684; DnaJ_CXXCXGXG 

SMART

 SM00271; DnaJ 

PROSITE

 PS00636; DNAJ_1
 PS50076; DNAJ_2
 PS51188; ZF_CR 

PRINTS

 PR00625; JDOMAIN.