UniProt Accession

 NELFE_HUMAN; P18615; A2BE08; Q5JP74; Q5JP75; Q96F56; Q9NPK2 

Genbank Protein ID

 M33231; M32274; M32275; M32276; M33230; L03411; AF019413; AL049547; AL662849; AL844853; BX005143; CR759782; CR388219; CR753845; AL645922; CR753845; CR388219; CH471081; BC025235; BC050617; X16105 

Genbank Nucleotide ID

 AAA36308.1; AAA36308.1; AAA36308.1; AAA36308.1; AAA36308.1; AAC37523.1; AAB67979.1; CAB89308.1; CAI17457.1; CAI41861.1; CAM25865.1; CAQ07114.1; CAQ07466.1; CAQ07466.1; CAQ09275.1; CAQ10906.1; CAQ10906.1; EAX03553.1; AAH25235.1; AAH50617.1; CAA34231.1 

Protein Name

 Negative elongation factor E 

Protein Synonyms/Alias

 NELF-E; RNA-binding protein RD 

Gene Name

 NELFE 

Gene Synonyms/Alias

 RD; RDBP 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
130	SSRRPQRKSLYESFV	ubiquitination	[1]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 

Functional Description

 Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. 

Sequence Annotation

 REPEAT 184 185 1.
 REPEAT 186 187 2.
 REPEAT 188 189 3.
 REPEAT 190 191 4.
 REPEAT 192 193 5; approximate.
 REPEAT 194 195 6.
 REPEAT 196 197 7.
 REPEAT 198 199 8.
 REPEAT 200 201 9.
 REPEAT 202 203 10.
 REPEAT 204 205 11.
 REPEAT 206 207 12.
 REPEAT 208 209 13.
 REPEAT 210 211 14.
 REPEAT 212 213 15.
 REPEAT 214 215 16.
 REPEAT 216 217 17.
 REPEAT 218 219 18.
 REPEAT 220 221 19.
 REPEAT 222 223 20.
 REPEAT 224 225 21.
 REPEAT 226 227 22.
 REPEAT 228 229 23.
 REPEAT 230 231 24.
 REPEAT 232 233 25.
 REPEAT 234 235 26.
 REPEAT 236 237 27.
 REPEAT 238 239 28.
 REPEAT 240 241 29.
 REPEAT 242 243 30.
 DOMAIN 262 332 RRM.
 REGION 184 243 30 X 2 AA approximate tandem repeats of
 MOD_RES 51 51 Phosphoserine (By similarity).
 MOD_RES 113 113 Phosphoserine.
 MOD_RES 115 115 Phosphoserine.
 MOD_RES 131 131 Phosphoserine.
 MOD_RES 181 181 Phosphoserine.
 MOD_RES 185 185 Phosphoserine.
 MOD_RES 251 251 Phosphoserine.
 MOD_RES 272 272 Phosphothreonine.
 MOD_RES 274 274 Phosphothreonine.
 MOD_RES 281 281 Phosphoserine.
 MOD_RES 353 353 Phosphoserine.  

Keyword

 3D-structure; Coiled coil; Complete proteome; Direct protein sequencing; Nucleus; Phosphoprotein; Reference proteome; Repeat; Repressor; RNA-binding; Transcription; Transcription regulation; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 380 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; IDA:HPA.
 GO:0032021; C:NELF complex; IDA:UniProtKB.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0003723; F:RNA binding; NAS:UniProtKB.
 GO:0034244; P:negative regulation of transcription elongation from RNA polymerase II promoter; IEA:Compara.
 GO:0050434; P:positive regulation of viral transcription; TAS:Reactome.
 GO:0006368; P:transcription elongation from RNA polymerase II promoter; TAS:Reactome.
 GO:0022415; P:viral reproductive process; TAS:Reactome. 

Interpro

 IPR012677; Nucleotide-bd_a/b_plait.
 IPR000504; RRM_dom. 

Pfam

 PF00076; RRM_1 

SMART

 SM00360; RRM 

PROSITE

 PS50102; RRM 

PRINTS