CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019654
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 CDK5 regulatory subunit-associated protein 1 
Protein Synonyms/Alias
 CDK5 activator-binding protein C42 
Gene Name
 CDK5RAP1 
Gene Synonyms/Alias
 C20orf34; CGI-05; HSPC167 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
70PTFQHFLKSASAPQEubiquitination[1, 2, 3, 4]
78SASAPQEKLSSEVEDubiquitination[2]
189GCMAERLKEEILNREubiquitination[2]
197EEILNREKMVDILAGubiquitination[2]
287ASILEEVKKLSEQVFubiquitination[2]
378RFTSPHPKDFPDEVLubiquitination[1, 2, 4]
396HERDNICKQIHLPAQubiquitination[2]
487TRAYHRLKDDVPEEVubiquitination[2]
495DDVPEEVKLRRLEELubiquitination[2]
546GRNDGNLKVIFPDAEubiquitination[2]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Specifically inhibits CDK5 activation by CDK5R1. 
Sequence Annotation
 DOMAIN 100 220 MTTase N-terminal.
 DOMAIN 515 590 TRAM.
 REGION 22 157 CDK5 activation inhibition.
 REGION 475 586 CDK5R1-binding.
 METAL 109 109 Iron-sulfur (4Fe-4S) (By similarity).
 METAL 145 145 Iron-sulfur (4Fe-4S) (By similarity).
 METAL 183 183 Iron-sulfur (4Fe-4S) (By similarity).
 METAL 258 258 Iron-sulfur (4Fe-4S-S-AdoMet) (By
 METAL 262 262 Iron-sulfur (4Fe-4S-S-AdoMet) (By
 METAL 265 265 Iron-sulfur (4Fe-4S-S-AdoMet) (By  
Keyword
 4Fe-4S; Alternative splicing; Complete proteome; Iron; Iron-sulfur; Metal-binding; Reference proteome; S-adenosyl-L-methionine. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 601 AA 
Protein Sequence
MHPLQCVLQV QRSLGWGPLA SVSWLSLRMC RAHSSLSSTM CPSPERQEDG ARKDFSSRLA 60
AGPTFQHFLK SASAPQEKLS SEVEDPPPYL MMDELLGRQR KVYLETYGCQ MNVNDTEIAW 120
SILQKSGYLR TSNLQEADVI LLVTCSIREK AEQTIWNRLH QLKALKTRRP RSRVPLRIGI 180
LGCMAERLKE EILNREKMVD ILAGPDAYRD LPRLLAVAES GQQAANVLLS LDETYADVMP 240
VQTSASATSA FVSIMRGCDN MCSYCIVPFT RGRERSRPIA SILEEVKKLS EQVFLPPRPP 300
KVLGLQGLKE VTLLGQNVNS FRDNSEVQFN SAVPTNLSRG FTTNYKTKQG GLRFAHLLDQ 360
VSRVDPEMRI RFTSPHPKDF PDEVLQLIHE RDNICKQIHL PAQSGSSRVL EAMRRGYSRE 420
AYVELVHHIR ESIPGVSLSS DFIAGFCGET EEDHVQTVSL LREVQYNMGF LFAYSMRQKT 480
RAYHRLKDDV PEEVKLRRLE ELITIFREEA TKANQTSVGC TQLVLVEGLS KRSATDLCGR 540
NDGNLKVIFP DAEMEDVNNP GLRVRAQPGD YVLVKITSAS SQTLRGHVLC RTTLRDSSAY 600
C 601 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:InterPro.
 GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0019901; F:protein kinase binding; NAS:UniProtKB.
 GO:0016740; F:transferase activity; IEA:InterPro.
 GO:0007420; P:brain development; NAS:UniProtKB.
 GO:0045736; P:negative regulation of cyclin-dependent protein serine/threonine kinase activity; IDA:UniProtKB.
 GO:0045664; P:regulation of neuron differentiation; NAS:UniProtKB.
 GO:0006400; P:tRNA modification; IEA:InterPro. 
Interpro
 IPR006638; Elp3/MiaB/NifB.
 IPR023970; MeThioTfrase/rSAM.
 IPR005839; Methylthiotransferase.
 IPR020612; Methylthiotransferase_CS.
 IPR013848; Methylthiotransferase_N.
 IPR006463; MiaB_methiolase.
 IPR007197; rSAM.
 IPR023404; rSAM_horseshoe.
 IPR002792; TRAM_dom. 
Pfam
 PF04055; Radical_SAM
 PF01938; TRAM
 PF00919; UPF0004 
SMART
 SM00729; Elp3 
PROSITE
 PS51449; MTTASE_N
 PS01278; MTTASE_RADICAL
 PS50926; TRAM 
PRINTS