UniProt Accession

 KCC2A_RAT; P11275 

Genbank Protein ID

 J02942; M16960; M29699 

Genbank Nucleotide ID

 AAA41870.1; AAA41855.1; AAA40841.1 

Protein Name

 Calcium/calmodulin-dependent protein kinase type II subunit alpha 

Protein Synonyms/Alias

 CaM kinase II subunit alpha; CaMK-II subunit alpha 

Gene Name

 Camk2a 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
42	AGQEYAAKIINTKKL	ubiquitination	[1]
68	ARICRLLKHPNIVRL	ubiquitination	[1]
146	ENLLLASKLKGAAVK	acetylation	[2]
146	ENLLLASKLKGAAVK	ubiquitination	[1]
250	EAKDLINKMLTINPS	ubiquitination	[1]
258	MLTINPSKRITAAEA	ubiquitination	[1]
267	ITAAEALKHPWISHR	ubiquitination	[1]
328	NKKNDGVKESSESTN	ubiquitination	[1]
344	TIEDEDTKVRKQEII	ubiquitination	[1]
461	VWHRRDGKWQIVHFH	acetylation	[2]

Reference

 [1] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113]
 [2] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. 

Sequence Annotation

 DOMAIN 13 271 Protein kinase.
 NP_BIND 19 27 ATP (By similarity).
 REGION 290 300 Calmodulin-binding.
 REGION 310 320 Interaction with BAALC.
 ACT_SITE 135 135 Proton acceptor.
 BINDING 42 42 ATP (By similarity).
 MOD_RES 13 13 Phosphotyrosine (By similarity).
 MOD_RES 25 25 Phosphoserine (By similarity).
 MOD_RES 275 275 Phosphoserine (By similarity).
 MOD_RES 286 286 Phosphothreonine; by autocatalysis.
 MOD_RES 333 333 Phosphoserine (By similarity).
 MOD_RES 334 334 Phosphothreonine (By similarity).
 MOD_RES 337 337 Phosphothreonine (By similarity).  

Keyword

 3D-structure; ATP-binding; Calmodulin-binding; Cell junction; Cell membrane; Complete proteome; Direct protein sequencing; Kinase; Membrane; Nucleotide-binding; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Synapse; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 478 AA 

Protein Sequence

Gene Ontology

 GO:0005954; C:calcium- and calmodulin-dependent protein kinase complex; TAS:UniProtKB.
 GO:0030054; C:cell junction; IEA:UniProtKB-KW.
 GO:0032839; C:dendrite cytoplasm; IDA:BHF-UCL.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-KW.
 GO:0042734; C:presynaptic membrane; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004683; F:calmodulin-dependent protein kinase activity; IDA:RGD.
 GO:0035235; P:ionotropic glutamate receptor signaling pathway; IDA:UniProtKB.
 GO:0018105; P:peptidyl-serine phosphorylation; IDA:RGD.
 GO:0046777; P:protein autophosphorylation; IDA:RGD. 

Interpro

 IPR020636; Ca/CaM-dep_Ca-dep_prot_Kinase.
 IPR013543; Ca/CaM-dep_prot_kinase-assoc.
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 

Pfam

 PF08332; CaMKII_AD
 PF00069; Pkinase 

SMART

 SM00220; S_TKc 

PROSITE

 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 

PRINTS