CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-045115
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Ubiquitin carboxyl-terminal hydrolase 
Protein Synonyms/Alias
  
Gene Name
 USP32 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
621EKYTGLKKQLSDLCGubiquitination[1, 2]
832FTLRVVQKDGNSCAWubiquitination[3]
1207YAKNPNCKWYCYNDSubiquitination[4, 5]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Hydrolase; Protease; Reference proteome; Thiol protease; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1274 AA 
Protein Sequence
MGHLTLEDYQ IWSVKNVLAN EFLNLLFQVC HIVLGLRPAT PEEEGQIIRG WLERESRYGL 60
QAGHNWFIIS MQWWQQWKEY VKYDANPVVI EPSSVLNGGK YSFGTAAHPM EQVEDRIGSS 120
LSYVNTTEEK FSDNISTASE ASETAGSGFL YSATPGADVC FARQHNTSDN NNQCLLGANG 180
NILLHLNPQK PGAIDNQPLV TQEPVKATSL TLEGGRLKRT PQLIHGRDYE MVPEPVWRAL 240
YHWYGANLAL PRPVIKNSKT DIPELELFPR YLLFLRQQPA TRTQQSNIWV NMGNVPSPNA 300
PLKRVLAYTG CFSRMQTIKE IHEYLSQRLR IKEEDMRLWL YNSENYLTLL DDEDHKLEYL 360
KIQDEQHLVI EVRNKDMSWP EEMSFIANSS KIDRHKVPTE KGATGLSNLG NTCFMNSSIQ 420
CVSNTQPLTQ YFISGRHLYE LNRTNPIGMK GHMAKCYGDL VQELWSGTQK NVAPLKLRWT 480
IAKYAPRFNG FQQQDSQELL AFLLDGLHED LNRVHEKPYV ELKDSDGRPD WEVAAEAWDN 540
HLRRNRSIVV DLFHGQLRSQ VKCKTCGHIS VRFDPFNFLS LPLPMDSYMH LEITVIKLDG 600
TTPVRYGLRL NMDEKYTGLK KQLSDLCGLN SEQILLAEVH GSNIKNFPQD NQKVRLSVSG 660
FLCAFEIPVP VSPISASSPT QTDFSSSPST NEMFTLTTNG DLPRPIFIPN GMPNTVVPCG 720
TEKNFTNGMV NGHMPSLPDS PFTGYIIAVH RKMMRTELYF LSSQKNRPSL FGMPLIVPCT 780
VHTRKKDLYD AVWIQVSRLA SPLPPQEASN HAQDCDDSMG YQYPFTLRVV QKDGNSCAWC 840
PWYRFCRGCK IDCGEDRAFI GNAYIAVDWD PTALHLRYQT SQERVVDEHE SVEQSRRAQA 900
EPINLDSCLR AFTSEEELGE NEMYYCSKCK THCLATKKLD LWRLPPILII HLKRFQFVNG 960
RWIKSQKIVK FPRESFDPSA FLVPRDPALC QHKPLTPQGD ELSEPRILAR EVKKVDAQSS 1020
AGEEDVLLSK SPSSLSANII SSPKGSPSSS RKSGTSCPSS KNSSPNSSPR TLGRSKGRLR 1080
LPQIGSKNKL SSSKENLDAS KENGAGQICE LADALSRGHV LGGSQPELVT PQDHEVALAN 1140
GFLYEHEACG NGYSNGQLGN HSEEDSTDDQ REDTRIKPIY NLYAISCHSG ILGGGHYVTY 1200
AKNPNCKWYC YNDSSCKELH PDEIDTDSAY ILFYEQQGID YAQFLPKTDG KKMADTSSMD 1260
EDFESDYKKY CVLQ 1274 
Gene Ontology
 GO:0008234; F:cysteine-type peptidase activity; IEA:UniProtKB-KW.
 GO:0004221; F:ubiquitin thiolesterase activity; IEA:InterPro.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro. 
Interpro
 IPR006615; Pept_C19_DUSP.
 IPR018200; Pept_C19ubi-hydrolase_C_CS.
 IPR001394; Peptidase_C19. 
Pfam
 PF06337; DUSP
 PF00443; UCH 
SMART
 SM00695; DUSP 
PROSITE
 PS51283; DUSP
 PS00972; UCH_2_1
 PS00973; UCH_2_2
 PS50235; UCH_2_3 
PRINTS