CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016477
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Aspartate--tRNA ligase, mitochondrial 
Protein Synonyms/Alias
 Aspartyl-tRNA synthetase; AspRS 
Gene Name
 Dars2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
376ICVHDGAKYLRKEDIacetylation[1]
Reference
 [1] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123] 
Functional Description
  
Sequence Annotation
 MOD_RES 218 218 Phosphothreonine (By similarity).  
Keyword
 Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Ligase; Mitochondrion; Nucleotide-binding; Phosphoprotein; Protein biosynthesis; Reference proteome; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 653 AA 
Protein Sequence
MYLGFWLSRL CRGLSRPIGK TMRPIWGSLS RNLALSSQRI PEFSSFVART NTCGELRSSH 60
LGQEVTLCGW IQYRRQNTFL VLRDCHGLVQ ILIPQDESAA SVRRILCEAP VESVVRVSGT 120
VISRPPGQEN PKMPTGEIEI KVKTAELLNA CKKLPFEIKD FVKKTEALRL QYRYLDLRSF 180
QMQYNLRLRS QMVMKMREYL CNLHGFVDIE TPTLFKRTPG GAKEFLVPSR EPGKFYSLPQ 240
SPQQFKQLLM VGGLDRYFQV ARCYRDEGSR PDRQPEFTQI DIEMSFVEQT GIQRLVEGLL 300
QYSWPGDKDP LVTPFPSMTF AEALATYGTD KPDTRFGMKI VDVSDVFRNT ELRFLQDALA 360
KPQGTVKAIC VHDGAKYLRK EDIEFIRKFA VHHFSQEVLP IFLNAKKNWS SPFAKFIMEE 420
ERLELARSME IQEEDIVLLT AGEHEKACSL LGKLRLECAD LLEMRGAVLR DPAVFSFLWV 480
VDFPLFLAKE ESPTELESAH HPFTAPNSSD IHLLYTEPEK VRGQHYDLVL NGNEIGGGSV 540
RIHDAQLQRY ILETLLKEDV KLLSHLLQAL DYGAPPHGGI ALGLDRLVCL VTGAPSIRDV 600
IAFPKSYRGQ DLMSNAPDSV SPEELKPYHI HVLWPADSEE ESASATPSKH LSS 653 
Gene Ontology
 GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0005634; C:nucleus; IEA:Compara.
 GO:0004815; F:aspartate-tRNA ligase activity; IEA:EC.
 GO:0050560; F:aspartate-tRNA(Asn) ligase activity; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0003676; F:nucleic acid binding; IEA:InterPro.
 GO:0006418; P:tRNA aminoacylation for protein translation; IEA:InterPro. 
Interpro
 IPR004364; aa-tRNA-synt_II.
 IPR018150; aa-tRNA-synt_II-like.
 IPR006195; aa-tRNA-synth_II.
 IPR004524; Asp-tRNA-ligase_IIb_bac/mt.
 IPR002312; Asp/Asn-tRNA-synth_IIb.
 IPR004115; GAD_dom.
 IPR012340; NA-bd_OB-fold.
 IPR004365; NA-bd_OB_tRNA-helicase. 
Pfam
 PF02938; GAD
 PF00152; tRNA-synt_2
 PF01336; tRNA_anti 
SMART
  
PROSITE
 PS50862; AA_TRNA_LIGASE_II 
PRINTS
 PR01042; TRNASYNTHASP.