UniProt Accession

 NIT1_MOUSE; Q8VDK1; O88526; Q9R1N4 

Genbank Protein ID

 AF069985; AF069988; BC021634 

Genbank Nucleotide ID

 AAC40184.1; AAC40185.1; AAH21634.1 

Protein Name

 Nitrilase homolog 1 

Protein Synonyms/Alias

  

Gene Name

 Nit1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
157	SVVASYRKTHLCDVE	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Plays a role in cell growth and apoptosis: loss of expression promotes cell growth, resistance to DNA damage stress and increased incidence to NMBA-induced tumors. Has tumor suppressor properties that enhances the apoptotic responsiveness in cancer cells; this effect is additive to the tumor suppressor activity of FHIT. It is also a negative regulator of primary T- cells. Has apparently no omega-amidase activity such as NIT2. 

Sequence Annotation

 DOMAIN 43 316 CN hydrolase.
 ACT_SITE 82 82 Proton acceptor (Potential).
 ACT_SITE 157 157 By similarity.
 ACT_SITE 199 199 Nucleophile (By similarity).  

Keyword

 Alternative splicing; Complete proteome; Cytoplasm; Hydrolase; Mitochondrion; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 323 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0016810; F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds; IEA:InterPro.
 GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro. 

Interpro

 IPR003010; C-N_Hydrolase.
 IPR001110; UPF0012_CS. 

Pfam

 PF00795; CN_hydrolase 

SMART

  

PROSITE

 PS50263; CN_HYDROLASE
 PS01227; UPF0012 

PRINTS