| Tag | Content |
|---|
| CPLM ID | CPLM-011231 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Arginine biosynthesis bifunctional protein ArgJ, mitochondrial |
Protein Synonyms/Alias | Extracellular mutant protein 40; Glutamate N-acetyltransferase; GAT; Ornithine acetyltransferase; OATase; Ornithine transacetylase; Amino-acid acetyltransferase; N-acetylglutamate synthase; AGS; Arginine biosynthesis bifunctional protein ArgJ alpha chain; Arginine biosynthesis bifunctional protein ArgJ beta chain |
Gene Name | ARG7 |
Gene Synonyms/Alias | ECM40; YMR062C; YM9916.01C |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 24 | KYALYVPKTGSFPKG | acetylation | [1] | | 90 | VLETARGKNINAIVV | acetylation | [1] | | 147 | GQRLQMDKISTGINK | acetylation | [1] | | 154 | KISTGINKIFGEEKF | acetylation | [1] | | 190 | KLVTSRFKLPSGTEY | acetylation | [1] | | 233 | IESKALQKMLTFATT | acetylation | [1] | | 276 | KEINEDSKDFEQVKL | acetylation | [1] | | 355 | LCAIGYAKLNDLKSL | acetylation | [1] | | 360 | YAKLNDLKSLDVNKI | acetylation | [1] |
|
Reference | [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae. Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C. Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [ PMID: 22865919] |
Functional Description | Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of acetylglutamate from glutamate and acetyl-CoA, and of ornithine by transacetylation between acetylornithine and glutamate. |
Sequence Annotation | ACT_SITE 215 215 Nucleophile (By similarity).
BINDING 177 177 Substrate (By similarity).
BINDING 204 204 Substrate (By similarity).
BINDING 215 215 Substrate (By similarity).
BINDING 301 301 Substrate (By similarity).
BINDING 436 436 Substrate (By similarity).
BINDING 441 441 Substrate (By similarity). |
Keyword | Acyltransferase; Amino-acid biosynthesis; Arginine biosynthesis; Autocatalytic cleavage; Complete proteome; Direct protein sequencing; Mitochondrion; Multifunctional enzyme; Reference proteome; Transferase; Transit peptide. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 441 AA |
Protein Sequence | MRISSTLLQR SKQLIDKYAL YVPKTGSFPK GFEVGYTASG VKKNGSLDLG VILNTNKSRP 60
STAAAVFTTN KFKAAPVLTS KKVLETARGK NINAIVVNSG CANSVTGDLG MKDAQVMIDL 120
VNDKIGQKNS TLVMSTGVIG QRLQMDKIST GINKIFGEEK FGSDFNSWLN VAKSICTTDT 180
FPKLVTSRFK LPSGTEYTLT GMAKGAGMIC PNMATLLGFI VTDLPIESKA LQKMLTFATT 240
RSFNCISVDG DMSTNDTICM LANGAIDTKE INEDSKDFEQ VKLQVTEFAQ RLAQLVVRDG 300
EGSTKFVTVN VKNALHFEDA KIIAESISNS MLVKTALYGQ DANWGRILCA IGYAKLNDLK 360
SLDVNKINVS FIATDNSEPR ELKLVANGVP QLEIDETRAS EILALNDLEV SVDLGTGDQA 420
AQFWTCDLSH EYVTINGDYR S 441 |
Gene Ontology | GO:0005759; C:mitochondrial matrix; IDA:SGD. GO:0004042; F:acetyl-CoA:L-glutamate N-acetyltransferase activity; IDA:SGD. GO:0004358; F:glutamate N-acetyltransferase activity; IDA:SGD. GO:0006526; P:arginine biosynthetic process; TAS:SGD. GO:0006592; P:ornithine biosynthetic process; IDA:SGD. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |