CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007912
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Actin-related protein 3 
Protein Synonyms/Alias
 Actin-like protein ARP3; Actin-like protein 3 
Gene Name
 ARP3 
Gene Synonyms/Alias
 ACT4; YJR065C; J1760 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
87LSNNLSGKRGTEDLDubiquitination[1]
137FWENSIFKYLRTEPEubiquitination[1]
282KEFNKFDKDPSKFAQubiquitination[1]
286KFDKDPSKFAQFVVEacetylation[2]
301NQEKTRRKVVDIGYEacetylation[2]
391SELLSGTKSTGVDVSubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 
Functional Description
 Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament (By similarity). 
Sequence Annotation
  
Keyword
 Actin-binding; ATP-binding; Complete proteome; Cytoplasm; Cytoskeleton; Nucleotide-binding; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 449 AA 
Protein Sequence
MSYLNNPAVV MDNGTGLTKL GFAGNDSPSW VFPTAIATAA PSNTKKSSGV GAPSAVSNEA 60
SYFGNSTSAT NFNGATGGLL SNNLSGKRGT EDLDFYIGNE ALVASQGPSY SLSYPIRHGQ 120
VENWDHMERF WENSIFKYLR TEPEDHFFLL TEPPLNPPEN REQVAEIFFE SFNCAGLYIA 180
VQAVLALAAS WTSSKVTDRS LTGTVIDSGD GVTHVIPVAE GYVIGSAIKN IPIAGRDITL 240
FIQSLLRERG EADTSLRTAE KIKQEYCYVC PDIVKEFNKF DKDPSKFAQF VVENQEKTRR 300
KVVDIGYERF LAPEIFFNPE IASSDFLTPL PTVVDQTIQA CPIDVRKGLY NNIVLSGGST 360
MFKDFGRRLQ RDLKSIVNNR IAQSELLSGT KSTGVDVSVI SHRKQRNAVW FGGSLLAQTA 420
EFKGYCHTKK DYEEYGPEIV RNFSLFNMV 449 
Gene Ontology
 GO:0030479; C:actin cortical patch; IEA:UniProtKB-SubCell.
 GO:0005885; C:Arp2/3 protein complex; IDA:SGD.
 GO:0005886; C:plasma membrane; IDA:SGD.
 GO:0005524; F:ATP binding; IDA:SGD.
 GO:0034314; P:Arp2/3 complex-mediated actin nucleation; IMP:SGD.
 GO:0000001; P:mitochondrion inheritance; TAS:SGD. 
Interpro
 IPR004000; Actin-related.
 IPR020902; Actin/actin-like_CS.
 IPR015623; Arp3. 
Pfam
 PF00022; Actin 
SMART
 SM00268; ACTIN 
PROSITE
 PS01132; ACTINS_ACT_LIKE 
PRINTS